2ovg
From Proteopedia
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| - | [[Image:2ovg.jpg|left|200px]] | + | [[Image:2ovg.jpg|left|200px]] |
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| - | '''Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221''' | + | {{Structure |
| + | |PDB= 2ovg |SIZE=350|CAPTION= <scene name='initialview01'>2ovg</scene>, resolution 1.350Å | ||
| + | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+111'>AC1</scene> and <scene name='pdbsite=AC2:Epe+Binding+Site+For+Residue+A+110'>AC2</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID'>EPE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= cro ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | ||
| + | }} | ||
| + | |||
| + | '''Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OVG is a [ | + | 2OVG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OVG OCA]. |
==Reference== | ==Reference== | ||
| - | Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA., Hall BM, Roberts SA, Heroux A, Cordes MH, J Mol Biol. 2008 Jan 18;375(3):802-11. Epub 2007 Nov 6. PMID:[http:// | + | Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA., Hall BM, Roberts SA, Heroux A, Cordes MH, J Mol Biol. 2008 Jan 18;375(3):802-11. Epub 2007 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18054042 18054042] |
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:05:00 2008'' |
Revision as of 16:05, 20 March 2008
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| , resolution 1.350Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | and | ||||||
| Gene: | cro (Enterobacteria phage lambda) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Lambda Cro Q27P/A29S/K32Q triple mutant at 1.35 A in space group P3221
Overview
Previously reported crystal structures of free and DNA-bound dimers of lambda Cro differ strongly (about 4 A backbone rmsd), suggesting both flexibility of the dimer interface and induced-fit protein structure changes caused by sequence-specific DNA binding. Here, we present two crystal structures, in space groups P3(2)21 and C2 at 1.35 and 1.40 A resolution, respectively, of a variant of lambda Cro with three mutations in its recognition helix (Q27P/A29S/K32Q, or PSQ for short). One dimer structure (P3(2)21; PSQ form 1) resembles the DNA-bound wild-type Cro dimer (1.0 A backbone rmsd), while the other (C2; PSQ form 2) resembles neither unbound (3.6 A) nor bound (2.4 A) wild-type Cro. Both PSQ form 2 and unbound wild-type dimer crystals have a similar interdimer beta-sheet interaction between the beta1 strands at the edges of the dimer. In the former, an infinite, open beta-structure along one crystal axis results, while in the latter, a closed tetrameric barrel is formed. Neither the DNA-bound wild-type structure nor PSQ form 1 contains these interdimer interactions. We propose that beta-sheet superstructures resulting from crystal contact interactions distort Cro dimers from their preferred solution conformation, which actually resembles the DNA-bound structure. These results highlight the remarkable flexibility of lambda Cro but also suggest that sequence-specific DNA binding may not induce large changes in the protein structure.
About this Structure
2OVG is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA., Hall BM, Roberts SA, Heroux A, Cordes MH, J Mol Biol. 2008 Jan 18;375(3):802-11. Epub 2007 Nov 6. PMID:18054042
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