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3x00
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(Difference between revisions)
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| - | ''' | + | ==Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution== |
| + | <StructureSection load='3x00' size='340' side='right' caption='[[3x00]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3x00]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X00 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X00 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDN:ETHANE-1,2-DIAMINE'>EDN</scene>, <scene name='pdbligand=ZOF:6-({5-[(2E,3AS,4S,6AR)-2-IMINOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}AMINO)HEXANOIC+ACID'>ZOF</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wzn|3wzn]], [[3wzo|3wzo]], [[3wzp|3wzp]], [[3wzq|3wzq]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x00 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3x00 RCSB], [http://www.ebi.ac.uk/pdbsum/3x00 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein. | ||
| - | + | Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant.,Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A Biosci Biotechnol Biochem. 2015 Jan 3:1-3. PMID:25560769<ref>PMID:25560769</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | + | </StructureSection> | |
| + | [[Category: Doi, H]] | ||
| + | [[Category: Inoue, T]] | ||
[[Category: Kanai, M]] | [[Category: Kanai, M]] | ||
| - | [[Category: | + | [[Category: Kawato, T]] |
| - | + | ||
| - | + | ||
| - | + | ||
[[Category: Kodama, T]] | [[Category: Kodama, T]] | ||
| + | [[Category: Matsumura, H]] | ||
[[Category: Matsuoka, M]] | [[Category: Matsuoka, M]] | ||
| - | [[Category: Matsumura, H]] | ||
| - | [[Category: Kawato, T]] | ||
[[Category: Meshizuka, T]] | [[Category: Meshizuka, T]] | ||
| + | [[Category: Mizohata, E]] | ||
| + | [[Category: Shimizu, Y]] | ||
| + | [[Category: Sugiyama, A]] | ||
[[Category: Takasu, N]] | [[Category: Takasu, N]] | ||
| + | [[Category: Yamamoto, T]] | ||
| + | [[Category: Beta-barrel]] | ||
| + | [[Category: Biotin binding protein]] | ||
Revision as of 16:47, 21 January 2015
Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution
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Categories: Doi, H | Inoue, T | Kanai, M | Kawato, T | Kodama, T | Matsumura, H | Matsuoka, M | Meshizuka, T | Mizohata, E | Shimizu, Y | Sugiyama, A | Takasu, N | Yamamoto, T | Beta-barrel | Biotin binding protein
