2owm
From Proteopedia
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| - | [[Image:2owm.jpg|left|200px]] | + | [[Image:2owm.jpg|left|200px]] |
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| - | '''Motor domain of Neurospora crassa kinesin-3 (NcKin3)''' | + | {{Structure |
| + | |PDB= 2owm |SIZE=350|CAPTION= <scene name='initialview01'>2owm</scene>, resolution 3.25Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+B+1001'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Residue+C+1001'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+D+1001'>AC4</scene>, <scene name='pdbsite=AC5:Adp+Binding+Site+For+Residue+A+1002'>AC5</scene>, <scene name='pdbsite=AC6:Adp+Binding+Site+For+Residue+B+1002'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Residue+C+1002'>AC7</scene> and <scene name='pdbsite=AC8:Adp+Binding+Site+For+Residue+D+1002'>AC8</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Plus-end-directed_kinesin_ATPase Plus-end-directed kinesin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.4 3.6.4.4] | ||
| + | |GENE= 17E5.250 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa]) | ||
| + | }} | ||
| + | |||
| + | '''Motor domain of Neurospora crassa kinesin-3 (NcKin3)''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OWM is a [ | + | 2OWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWM OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Feb 19;47(7):1848-1861. Epub 2008 Jan 19. PMID:[http:// | + | X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Feb 19;47(7):1848-1861. Epub 2008 Jan 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18205396 18205396] |
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
[[Category: Plus-end-directed kinesin ATPase]] | [[Category: Plus-end-directed kinesin ATPase]] | ||
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[[Category: neck linker]] | [[Category: neck linker]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:05:28 2008'' |
Revision as of 16:05, 20 March 2008
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| , resolution 3.25Å | |||||||
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| Sites: | , , , , , , and | ||||||
| Ligands: | and | ||||||
| Gene: | 17E5.250 (Neurospora crassa) | ||||||
| Activity: | Plus-end-directed kinesin ATPase, with EC number 3.6.4.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Motor domain of Neurospora crassa kinesin-3 (NcKin3)
Overview
Neurospora crassa kinesin NcKin3 belongs to a unique fungal-specific subgroup of small Kinesin-3-related motor proteins. One of its functions appears to be the transport of mitochondria along microtubules. Here, we present the X-ray structure of a C-terminally truncated monomeric construct of NcKin3 comprising the motor domain and the neck linker, and a 3-D image reconstruction of this motor domain bound to microtubules, by cryoelectron microscopy. The protein contains Mg.ADP bound to the active site, yet the structure resembles an ATP-bound state. By comparison with structures of the Kinesin-3 motor Kif1A in different nucleotide states (Kikkawa, M. et al. (2001) Nature (London, U.K.) 411, 439-445), the NcKin3 structure corresponds to the AMPPCP complex of Kif1A rather than the AMPPNP complex. NcKin3-specific differences in the coordination of the nucleotide and asymmetric interactions between adjacent molecules in the crystal are discussed in the context of the unusual kinetics of the dimeric wild-type motor and the monomeric construct used for crystal structure analysis. The NcKin3 motor decorates microtubules at a stoichiometry of one head per alphabeta-tubulin heterodimer, thereby forming an axial periodicity of 8 nm. In spite of unusual extensions at the N-terminus and within flexible loops L2, L8a, and L12 (corresponding to the K-loop of monomeric kinesins), the microtubule binding geometry is similar to that of other members of the kinesin family.
About this Structure
2OWM is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
X-ray Structure and Microtubule Interaction of the Motor Domain of Neurospora crassa NcKin3, a Kinesin with Unusual Processivity(,)., Marx A, Muller J, Mandelkow EM, Woehlke G, Bouchet-Marquis C, Hoenger A, Mandelkow E, Biochemistry. 2008 Feb 19;47(7):1848-1861. Epub 2008 Jan 19. PMID:18205396
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