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2owo

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[[Image:2owo.gif|left|200px]]<br /><applet load="2owo" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2owo.gif|left|200px]]
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caption="2owo, resolution 2.300&Aring;" />
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'''Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate'''<br />
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{{Structure
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|PDB= 2owo |SIZE=350|CAPTION= <scene name='initialview01'>2owo</scene>, resolution 2.300&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_ligase_(NAD(+)) DNA ligase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.2 6.5.1.2]
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|GENE= ligA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2OWO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA_ligase_(NAD(+)) DNA ligase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.2 6.5.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWO OCA].
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2OWO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWO OCA].
==Reference==
==Reference==
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Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17466627 17466627]
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Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17466627 17466627]
[[Category: DNA ligase (NAD(+))]]
[[Category: DNA ligase (NAD(+))]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: protein/dna complex]]
[[Category: protein/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:23:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:05:30 2008''

Revision as of 16:05, 20 March 2008

Image:2owo.gif


PDB ID 2owo

Drag the structure with the mouse to rotate
, resolution 2.300Å
Ligands: , and
Gene: ligA (Escherichia coli)
Activity: DNA ligase (NAD(+)), with EC number 6.5.1.2
Coordinates: save as pdb, mmCIF, xml



Last Stop on the Road to Repair: Structure of E.coli DNA Ligase Bound to Nicked DNA-Adenylate


Overview

NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and essential for growth. Their distinctive substrate specificity and domain organization vis-a-vis human ATP-dependent ligases make them outstanding targets for anti-infective drug discovery. We report here the 2.3 A crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition. LigA envelopes the DNA within a protein clamp. Large protein domain movements and remodeling of the active site orchestrate progression through the three chemical steps of the ligation reaction. The structure inspires a strategy for inhibitor design.

About this Structure

2OWO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate., Nandakumar J, Nair PA, Shuman S, Mol Cell. 2007 Apr 27;26(2):257-71. PMID:17466627

Page seeded by OCA on Thu Mar 20 18:05:30 2008

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