2p6p
From Proteopedia
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| - | [[Image:2p6p.jpg|left|200px]] | + | [[Image:2p6p.jpg|left|200px]] |
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| - | '''X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2''' | + | {{Structure |
| + | |PDB= 2p6p |SIZE=350|CAPTION= <scene name='initialview01'>2p6p</scene>, resolution 1.88Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= urdGT2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1906 Streptomyces fradiae]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P6P is a [ | + | 2P6P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6P OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin., Mittler M, Bechthold A, Schulz GE, J Mol Biol. 2007 Sep 7;372(1):67-76. Epub 2007 Jun 9. PMID:[http:// | + | Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin., Mittler M, Bechthold A, Schulz GE, J Mol Biol. 2007 Sep 7;372(1):67-76. Epub 2007 Jun 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17640665 17640665] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Streptomyces fradiae]] | [[Category: Streptomyces fradiae]] | ||
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[[Category: x-ray-diffraction]] | [[Category: x-ray-diffraction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:09:24 2008'' |
Revision as of 16:09, 20 March 2008
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| , resolution 1.88Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | urdGT2 (Streptomyces fradiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2
Overview
The glycosyltransferase UrdGT2 from Streptomyces fradiae catalyzes the formation of a glycosidic C-C bond between a polyketide aglycone and D-olivose. The enyzme was expressed in Escherichia coli, purified and crystallized. Its structure was established by X-ray diffraction at 1.9 A resolution. It is the first structure of a C-glycosyltransferase. UrdGT2 belongs to the structural family GT-B of the glycosyltransferases and is likely to form a C(2)-symmetric dimer in solution. The binding structures of donor and acceptor substrates in five structurally homologous enzymes provided a clear and consistent guide for the substrate-binding structure in UrdGT2. The modeled substrate locations suggest the deeply buried Asp137 as the activator for C-C bond formation and explain the reaction. The putative model can be used to design mutations that change the substrate specificity. Such mutants are of great interest in overcoming the increasing danger of antibiotic resistance.
About this Structure
2P6P is a Protein complex structure of sequences from Streptomyces fradiae. Full crystallographic information is available from OCA.
Reference
Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin., Mittler M, Bechthold A, Schulz GE, J Mol Biol. 2007 Sep 7;372(1):67-76. Epub 2007 Jun 9. PMID:17640665
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