2p6u
From Proteopedia
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| - | [[Image:2p6u.jpg|left|200px]] | + | [[Image:2p6u.jpg|left|200px]] |
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| - | '''Apo structure of the Hel308 superfamily 2 helicase''' | + | {{Structure |
| + | |PDB= 2p6u |SIZE=350|CAPTION= <scene name='initialview01'>2p6u</scene>, resolution 3.14Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Apo structure of the Hel308 superfamily 2 helicase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2P6U is a [ | + | 2P6U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6U OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http:// | + | Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417] |
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: sf2 helicase]] | [[Category: sf2 helicase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:09:27 2008'' |
Revision as of 16:09, 20 March 2008
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| , resolution 3.14Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Apo structure of the Hel308 superfamily 2 helicase
Overview
To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.
About this Structure
2P6U is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417
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