2pfb
From Proteopedia
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| - | [[Image:2pfb.jpg|left|200px]] | + | [[Image:2pfb.jpg|left|200px]] |
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| - | '''Structure of oxidized OhrR from Xanthamonas campestris''' | + | {{Structure |
| + | |PDB= 2pfb |SIZE=350|CAPTION= <scene name='initialview01'>2pfb</scene>, resolution 1.930Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= ohrR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 Xanthomonas campestris]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of oxidized OhrR from Xanthamonas campestris''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PFB is a [ | + | 2PFB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFB OCA]. |
==Reference== | ==Reference== | ||
| - | Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:[http:// | + | Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18042459 18042459] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xanthomonas campestris]] | [[Category: Xanthomonas campestris]] | ||
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[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:12:18 2008'' |
Revision as of 16:12, 20 March 2008
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| , resolution 1.930Å | |||||||
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| Gene: | ohrR (Xanthomonas campestris) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of oxidized OhrR from Xanthamonas campestris
Overview
The Xanthomonas campestris transcription regulator OhrR contains a reactive cysteine residue (C22) that upon oxidation by organic hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue C127'. Such modification induces the expression of a peroxidase that reduces OHPs to their less toxic alcohols. Here, we describe the structures of reduced and OHP-oxidized OhrR, visualizing the structural mechanism of OHP induction. Reduced OhrR takes a canonical MarR family fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the disruption of the Y36'-C22-Y47' interaction network and dissection of helix alpha5, which then allows the 135 degrees rotation and 8.2 A translation of C127', formation of the C22-C127' disulphide bond, and alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These changes result in the 28 degrees rigid body rotations of each winged helix-turn-helix motif and DNA dissociation. Similar effector-induced rigid body rotations are expected for most MarR family members.
About this Structure
2PFB is a Single protein structure of sequence from Xanthomonas campestris. Full crystallographic information is available from OCA.
Reference
Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:18042459
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