2pf5
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2pf5.gif|left|200px]] | + | [[Image:2pf5.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of the Human TSG-6 Link Module''' | + | {{Structure |
| + | |PDB= 2pf5 |SIZE=350|CAPTION= <scene name='initialview01'>2pf5</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=2PE:NONAETHYLENE GLYCOL'>2PE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= TNFAIP6, TSG6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Human TSG-6 Link Module''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PF5 is a [ | + | 2PF5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PF5 OCA]. |
==Reference== | ==Reference== | ||
| - | Plasticity of the TSG-6 HA-binding loop and mobility in the TSG-6-HA complex revealed by NMR and X-ray crystallography., Higman VA, Blundell CD, Mahoney DJ, Redfield C, Noble ME, Day AJ, J Mol Biol. 2007 Aug 17;371(3):669-84. Epub 2007 Jun 2. PMID:[http:// | + | Plasticity of the TSG-6 HA-binding loop and mobility in the TSG-6-HA complex revealed by NMR and X-ray crystallography., Higman VA, Blundell CD, Mahoney DJ, Redfield C, Noble ME, Day AJ, J Mol Biol. 2007 Aug 17;371(3):669-84. Epub 2007 Jun 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17585936 17585936] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: link module; hyaluronan-binding domain; alpha/beta domain]] | [[Category: link module; hyaluronan-binding domain; alpha/beta domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:12:14 2008'' |
Revision as of 16:12, 20 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | TNFAIP6, TSG6 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Human TSG-6 Link Module
Overview
Tumour necrosis factor-stimulated gene-6 (TSG-6) is a glycosaminoglycan-binding protein expressed during inflammatory and inflammation-like processes. Previously NMR structures were calculated for the Link module of TSG-6 (Link_TSG6) in its free state and when bound to an octasaccharide of hyaluronan (HA(8)). Heparin was found to compete for HA binding even though it interacts at a site that is distinct from the HA-binding surface. Here we present crystallography data on the free protein, and (15)N NMR relaxation data for the uncomplexed and HA(8)-bound forms of Link_TSG6. Although the Link module is comparatively rigid overall, the free protein shows a high degree of mobility in the beta4/beta5 loop and at the Cys47-Cys68 disulfide bond, both of which are regions involved in HA binding. When bound to HA(8), this dynamic behaviour is dampened, but not eliminated, suggesting a degree of dynamic matching between the protein and sugar that may decrease the entropic penalty of complex formation. A further highly dynamic residue is Lys54, which is distant from the HA-binding site, but was previously shown to be involved in heparin binding. When HA is bound, Lys54 becomes less mobile, providing evidence for an allosteric effect linking the HA and heparin-binding sites. A mechanism is suggested involving the beta2-strand and alpha2-helix. The crystal structure of free Link_TSG6 contains five molecules in the asymmetric unit that are highly similar to the NMR structure and support the dynamic behaviour seen near the HA-binding site: they show little or no electron density for the beta4/beta5 loop and display multiple conformations for the Cys47-Cys68 disulfide bond. The crystal structures were used in docking calculations with heparin. An extended interface between a Link_TSG6 dimer and heparin 11-mer was identified that is in excellent agreement with previous mutagenesis and calorimetric data, providing the basis for further investigation of this interaction.
About this Structure
2PF5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Plasticity of the TSG-6 HA-binding loop and mobility in the TSG-6-HA complex revealed by NMR and X-ray crystallography., Higman VA, Blundell CD, Mahoney DJ, Redfield C, Noble ME, Day AJ, J Mol Biol. 2007 Aug 17;371(3):669-84. Epub 2007 Jun 2. PMID:17585936
Page seeded by OCA on Thu Mar 20 18:12:14 2008
