4rtb
From Proteopedia
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| - | ''' | + | ==X-ray structure of the FeFe-hydrogenase maturase HydG from Carboxydothermus hydrogenoformans== |
| + | <StructureSection load='4rtb' size='340' side='right' caption='[[4rtb]], [[Resolution|resolution]] 2.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4rtb]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RTB FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rtb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rtb RCSB], [http://www.ebi.ac.uk/pdbsum/4rtb PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the radical S-adenosyl-L-methionine (SAM) [FeFe]-hydrogenase maturase HydG involved in CN- /CO synthesis is characterized by two internal tunnels connecting its tyrosine-binding pocket with the external medium and the C-terminal Fe4 S4 cluster-containing region. A comparison with a tryptophan-bound NosL structure suggests that substrate binding causes the closing of the first tunnel and, along with mutagenesis studies, that tyrosine binds to HydG with its amino group well positioned for H-abstraction by SAM. In this orientation the dehydroglycine (DHG) fragment caused by tyrosine CalphaCbeta bond scission can readily migrate through the second tunnel towards the C-terminal domain where both CN- and CO are synthesized. Our HydG structure appears to be in a relaxed state with its C-terminal cluster CysX2 CysX22 Cys motif exposed to solvent. A rotation of this domain coupled to Fe4 S4 cluster assembly would bury its putatively reactive unique Fe ion thereby allowing it to interact with DHG. | ||
| - | + | Crystal Structure of HydG from Carboxydothermus hydrogenoformans: A Trifunctional [FeFe]-Hydrogenase Maturase.,Nicolet Y, Pagnier A, Zeppieri L, Martin L, Amara P, Fontecilla-Camps JC Chembiochem. 2014 Dec 10. doi: 10.1002/cbic.201402661. PMID:25504963<ref>PMID:25504963</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Amara, P]] | ||
| + | [[Category: Fontecilla-Camps, J C]] | ||
[[Category: Martin, L]] | [[Category: Martin, L]] | ||
[[Category: Nicolet, Y]] | [[Category: Nicolet, Y]] | ||
[[Category: Pagnier, A]] | [[Category: Pagnier, A]] | ||
| - | [[Category: Amara, P]] | ||
[[Category: Zeppieri, L]] | [[Category: Zeppieri, L]] | ||
| - | [[Category: | + | [[Category: Co/cn synthase]] |
| + | [[Category: Fefe-hydrogenase maturase]] | ||
| + | [[Category: Lyase]] | ||
| + | [[Category: Radical sam enzyme]] | ||
Revision as of 16:37, 28 January 2015
X-ray structure of the FeFe-hydrogenase maturase HydG from Carboxydothermus hydrogenoformans
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