4qgx
From Proteopedia
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| - | ''' | + | ==Crystal sturcture of the R132K:R111L:L121E mutant of Cellular Retinoic Acid Binding ProteinII complexed with a synthetic ligand (Merocyanine) at 1.47 angstrom resolution== |
| + | <StructureSection load='4qgx' size='340' side='right' caption='[[4qgx]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4qgx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QGX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QGX FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMC:(2E,4E,6E)-3-METHYL-6-(1,3,3-TRIMETHYL-1,3-DIHYDRO-2H-INDOL-2-YLIDENE)HEXA-2,4-DIENAL'>LMC</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qgw|4qgw]], [[4qgv|4qgv]], [[3fep|3fep]], [[4exz|4exz]], [[4eej|4eej]], [[3f8a|3f8a]], [[2g7b|2g7b]], [[4i9s|4i9s]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qgx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qgx RCSB], [http://www.ebi.ac.uk/pdbsum/4qgx PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/RABP2_HUMAN RABP2_HUMAN]] Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein reengineering of cellular retinoic acid binding protein II (CRABPII) has yielded a genetically addressable system, capable of binding a profluorophoric chromophore that results in fluorescent protein/chromophore complexes. These complexes exhibit far-red emission, with high quantum efficiencies and brightness and also exhibit excellent pH stability spanning the range of 2-11. In the course of this study, it became evident that single mutations of L121E and R59W were most effective in improving the fluorescent characteristics of CRABPII mutants as well as the kinetics of complex formation. The readily crystallizable nature of these proteins was invaluable to provide clues for the observed spectroscopic behavior that results from single mutation of key residues. | ||
| - | + | "Turn-On" Protein Fluorescence: In Situ Formation of Cyanine Dyes.,Yapici I, Lee KS, Berbasova T, Nosrati M, Jia X, Vasileiou C, Wang W, Santos EM, Geiger JH, Borhan B J Am Chem Soc. 2015 Jan 14. PMID:25534273<ref>PMID:25534273</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geiger, J H]] | ||
| + | [[Category: Nosrati, M]] | ||
[[Category: Yapici, I]] | [[Category: Yapici, I]] | ||
| - | [[Category: | + | [[Category: Fluorescent protein tag]] |
| - | [[Category: | + | [[Category: Merocyanine dyes for fluorescent protein labeling]] |
| + | [[Category: Merocyanine protonated schiff base iminium]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Protein engineering]] | ||
| + | [[Category: Protein fluorescence]] | ||
Revision as of 16:40, 28 January 2015
Crystal sturcture of the R132K:R111L:L121E mutant of Cellular Retinoic Acid Binding ProteinII complexed with a synthetic ligand (Merocyanine) at 1.47 angstrom resolution
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