2pft
From Proteopedia
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| - | [[Image:2pft.gif|left|200px]] | + | [[Image:2pft.gif|left|200px]] |
| - | + | ||
| - | '''The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst''' | + | {{Structure |
| + | |PDB= 2pft |SIZE=350|CAPTION= <scene name='initialview01'>2pft</scene>, resolution 2.250Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Exoc7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PFT is a [ | + | 2PFT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFT OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst., Moore BA, Robinson HH, Xu Z, J Mol Biol. 2007 Aug 10;371(2):410-21. Epub 2007 May 22. PMID:[http:// | + | The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst., Moore BA, Robinson HH, Xu Z, J Mol Biol. 2007 Aug 10;371(2):410-21. Epub 2007 May 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17583731 17583731] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:12:29 2008'' |
Revision as of 16:12, 20 March 2008
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| , resolution 2.250Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | Exoc7 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst
Overview
The exocyst is a eukaryotic tethering complex necessary for the fusion of exocytic vesicles with the plasma membrane. Its function in vivo is tightly regulated by interactions with multiple small GTPases. Exo70, one of the eight subunits of the exocyst, is important for the localization of the exocyst to the plasma membrane. It interacts with TC10 and Rho3 GTPases in mammals and yeast, respectively, and has been shown recently to bind to the actin-polymerization complex Arp2/3. Here, we present the crystal structure of Mus musculus Exo70 at 2.25 A resolution. Exo70 is composed of alpha-helices in a series of right-handed helix-turn-helix motifs organized into a long rod of length 170 A and width 35 A. Although the alpha-helical organization of this molecule is similar to that in Saccharomyces cerevisiae Exo70, major structural differences are observed on the surface of the molecule, at the domain boundaries, and in various loop structures. In particular, the C-terminal domain of M. musculus Exo70 adopts a new orientation relative to the N-terminal half not seen in S. cerevisiae Exo70 structures. Given the low level of sequence conservation within Exo70, this structure provides new insights into our understanding of many species-specific functions of the exocyst.
About this Structure
2PFT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst., Moore BA, Robinson HH, Xu Z, J Mol Biol. 2007 Aug 10;371(2):410-21. Epub 2007 May 22. PMID:17583731
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