2phy
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2phy.jpg|left|200px]] | + | [[Image:2phy.jpg|left|200px]] |
| - | + | ||
| - | '''PHOTOACTIVE YELLOW PROTEIN, DARK STATE (UNBLEACHED)''' | + | {{Structure |
| + | |PDB= 2phy |SIZE=350|CAPTION= <scene name='initialview01'>2phy</scene>, resolution 1.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''PHOTOACTIVE YELLOW PROTEIN, DARK STATE (UNBLEACHED)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2PHY is a [ | + | 2PHY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. This structure supersedes the now removed PDB entry 1PHY. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PHY OCA]. |
==Reference== | ==Reference== | ||
| - | 1.4 A structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore., Borgstahl GE, Williams DR, Getzoff ED, Biochemistry. 1995 May 16;34(19):6278-87. PMID:[http:// | + | 1.4 A structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore., Borgstahl GE, Williams DR, Getzoff ED, Biochemistry. 1995 May 16;34(19):6278-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7756254 7756254] |
[[Category: Halorhodospira halophila]] | [[Category: Halorhodospira halophila]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: light sensor for negative phototaxis]] | [[Category: light sensor for negative phototaxis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:13:11 2008'' |
Revision as of 16:13, 20 March 2008
| |||||||
| , resolution 1.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHOTOACTIVE YELLOW PROTEIN, DARK STATE (UNBLEACHED)
Overview
A photosensing protein directs light energy captured by its chromophore into a photocycle. The protein's structure must accommodate the photocycle and promote the resulting chemical or conformational changes that lead to signal transduction. The 1.4 A crystallographic structure of photoactive yellow protein, determined by multiple isomorphous replacement methods, provides the first view at atomic resolution of a protein with a photocycle. The alpha/beta fold, which differs from the original chain tracing, shows striking similarity to distinct parts of the signal transduction proteins profilin and the SH2 domain. In the dark state structure of photoactive yellow protein, the novel 4-hydroxycinnamyl chromophore, covalently attached to Cys69, is buried within the major hydrophobic core of the protein and is tethered at both ends by hydrogen bonds. In the active site, the yellow anionic form of the chromophore is stabilized by hydrogen bonds from the side chains of Tyr42 and buried Glu46 to the phenolic oxygen atom and by electrostatic complementarity with the positively charged guanidinium group of Arg52. Thr50 further interlocks Tyr42, Glu46, and Arg52 through a network of active site hydrogen bonds. Arg52, located in a concavity of the protein surface adjacent to the dominant patch of negative electrostatic potential, shields the chromophore from solvent and is positioned to form a gateway for the phototactic signal. Overall, the high-resolution structure of photoactive yellow protein supports a mechanism whereby electrostatic interactions create an active site poised for photon-induced rearrangements and efficient protein-mediated signal transduction.
About this Structure
2PHY is a Single protein structure of sequence from Halorhodospira halophila. This structure supersedes the now removed PDB entry 1PHY. Full crystallographic information is available from OCA.
Reference
1.4 A structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore., Borgstahl GE, Williams DR, Getzoff ED, Biochemistry. 1995 May 16;34(19):6278-87. PMID:7756254
Page seeded by OCA on Thu Mar 20 18:13:11 2008
