2pjh
From Proteopedia
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| - | [[Image:2pjh.jpg|left|200px]] | + | [[Image:2pjh.jpg|left|200px]] |
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| - | '''Strctural Model of the p97 N domain- npl4 UBD complex''' | + | {{Structure |
| + | |PDB= 2pjh |SIZE=350|CAPTION= <scene name='initialview01'>2pjh</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= p97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), npl4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''Strctural Model of the p97 N domain- npl4 UBD complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PJH is a [ | + | 2PJH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJH OCA]. |
==Reference== | ==Reference== | ||
| - | Detailed structural insights into the p97-Npl4-Ufd1 interface., Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S, J Biol Chem. 2007 Jul 20;282(29):21361-9. Epub 2007 May 9. PMID:[http:// | + | Detailed structural insights into the p97-Npl4-Ufd1 interface., Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S, J Biol Chem. 2007 Jul 20;282(29):21361-9. Epub 2007 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17491009 17491009] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: ufd1]] | [[Category: ufd1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:13:45 2008'' |
Revision as of 16:13, 20 March 2008
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| Gene: | p97 (Mus musculus), npl4 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Strctural Model of the p97 N domain- npl4 UBD complex
Overview
The AAA ATPase, p97, achieves its versatility through binding to a wide range of cofactor proteins that adapt it to different cellular functions. The heterodimer UN (comprising Ufd1 and Npl4) is an adaptor complex that recruits p97 for numerous tasks, many of which involve the ubiquitin pathway. Insights into the structural specificity of p97 for its UN adaptor are currently negligible. Here, we present the solution structure of the Npl4 "ubiquitin-like" domain (UBD), which adopts a beta-grasp fold with a 3(10) helical insert. Moreover we performed a chemical shift perturbation analysis of its binding surface with the p97 N domain. We assigned the backbone amides of the p97 N domain and probed both its reciprocal binding surface with Npl4 UBD and its interaction with the p97-binding region of Ufd1. NMR data recorded on a 400-kDa full-length UN-hexamer p97 complex reveals an identical mode of interaction. We calculated a structural model for the p97 N-Npl4 UBD complex, and a comparison with the p97-p47 adaptor complex reveals subtle differences in p97 adaptor recognition and specificity.
About this Structure
2PJH is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Detailed structural insights into the p97-Npl4-Ufd1 interface., Isaacson RL, Pye VE, Simpson P, Meyer HH, Zhang X, Freemont PS, Matthews S, J Biol Chem. 2007 Jul 20;282(29):21361-9. Epub 2007 May 9. PMID:17491009
Page seeded by OCA on Thu Mar 20 18:13:45 2008
Categories: Mus musculus | Protein complex | Freemont, P. | Isaacson, R. | Meyer, H H. | Pye, V E. | Simpson, S. | Zhang, X. | Aaa | Atpase | Npl4 | P97 | Protein binding | Transport protein | Ufd1
