2ply

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[[Image:2ply.gif|left|200px]]<br /><applet load="2ply" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ply.gif|left|200px]]
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caption="2ply, resolution 2.60&Aring;" />
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'''Structure of the mRNA binding fragment of elongation factor SelB in complex with SECIS RNA.'''<br />
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{{Structure
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|PDB= 2ply |SIZE=350|CAPTION= <scene name='initialview01'>2ply</scene>, resolution 2.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE= selB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1525 Moorella thermoacetica])
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}}
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'''Structure of the mRNA binding fragment of elongation factor SelB in complex with SECIS RNA.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2PLY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLY OCA].
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2PLY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLY OCA].
==Reference==
==Reference==
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Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17537456 17537456]
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Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17537456 17537456]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: winged helix]]
[[Category: winged helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:14:41 2008''

Revision as of 16:14, 20 March 2008

Image:2ply.gif


PDB ID 2ply

Drag the structure with the mouse to rotate
, resolution 2.60Å
Ligands: , , and
Gene: selB (Moorella thermoacetica)
Coordinates: save as pdb, mmCIF, xml



Structure of the mRNA binding fragment of elongation factor SelB in complex with SECIS RNA.


Overview

Elongation factor SelB is responsible for co-translational incorporation of selenocysteine (Sec) into proteins. The UGA stop codon is recoded as a Sec codon in the presence of a downstream mRNA hairpin. In prokaryotes, in addition to the EF-Tu-like N-terminal domains, a C-terminal extension containing four tandem winged-helix motifs (WH1-4) recognizes the mRNA hairpin. The 2.3-A resolution crystal structure of the Escherichia coli WH3/4 domains bound to mRNA with mutagenesis data reveal that the two WH motifs use the same structural elements to bind RNA. The structure together with the 2.6-A resolution structure of the WH1-4 domains from Moorella thermoacetica bound to RNA revealed that a salt bridge connecting WH2 to WH3 modules is disrupted upon mRNA binding. The results provide a structural basis for the molecular switch that may allow communication between tRNA and mRNA binding sites and illustrate how RNA acts as an activator of the switch. The structures show that tandem WH motifs not only provide an excellent scaffold for RNA binding but can also have an active role in the function of protein-RNA complexes.

About this Structure

2PLY is a Protein complex structure of sequences from Moorella thermoacetica. Full crystallographic information is available from OCA.

Reference

Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB., Soler N, Fourmy D, Yoshizawa S, J Mol Biol. 2007 Jul 20;370(4):728-41. Epub 2007 May 10. PMID:17537456

Page seeded by OCA on Thu Mar 20 18:14:41 2008

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