2pmp

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[[Image:2pmp.jpg|left|200px]]<br /><applet load="2pmp" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2pmp.jpg|left|200px]]
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caption="2pmp, resolution 2.300&Aring;" />
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'''Structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from the isoprenoid biosynthetic pathway of Arabidopsis thaliana'''<br />
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{{Structure
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|PDB= 2pmp |SIZE=350|CAPTION= <scene name='initialview01'>2pmp</scene>, resolution 2.300&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=C5P:CYTIDINE-5'-MONOPHOSPHATE'>C5P</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12]
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|GENE= ISPF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
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}}
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'''Structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from the isoprenoid biosynthetic pathway of Arabidopsis thaliana'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2PMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=C5P:'>C5P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_2,4-cyclodiphosphate_synthase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.12 4.6.1.12] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PMP OCA].
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2PMP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PMP OCA].
==Reference==
==Reference==
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Biosynthesis of isoprenoids in plants: structure of the 2C-methyl-D-erithrytol 2,4-cyclodiphosphate synthase from Arabidopsis thaliana. Comparison with the bacterial enzymes., Calisto BM, Perez-Gil J, Bergua M, Querol-Audi J, Fita I, Imperial S, Protein Sci. 2007 Sep;16(9):2082-8. Epub 2007 Jul 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17660251 17660251]
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Biosynthesis of isoprenoids in plants: structure of the 2C-methyl-D-erithrytol 2,4-cyclodiphosphate synthase from Arabidopsis thaliana. Comparison with the bacterial enzymes., Calisto BM, Perez-Gil J, Bergua M, Querol-Audi J, Fita I, Imperial S, Protein Sci. 2007 Sep;16(9):2082-8. Epub 2007 Jul 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17660251 17660251]
[[Category: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase]]
[[Category: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase]]
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: arabidopsis thaliana]]
[[Category: arabidopsis thaliana]]
[[Category: cmp]]
[[Category: cmp]]
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[[Category: isoprenoid-binding proteins]]
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[[Category: isoprenoid-binding protein]]
[[Category: lyase]]
[[Category: lyase]]
[[Category: mep pathway]]
[[Category: mep pathway]]
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[[Category: plant enzymes]]
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[[Category: plant enzyme]]
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[[Category: zinc ions]]
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[[Category: zinc ion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:14:56 2008''

Revision as of 16:14, 20 March 2008


PDB ID 2pmp

Drag the structure with the mouse to rotate
, resolution 2.300Å
Ligands: , , and
Gene: ISPF (Arabidopsis thaliana)
Activity: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, with EC number 4.6.1.12
Coordinates: save as pdb, mmCIF, xml



Structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from the isoprenoid biosynthetic pathway of Arabidopsis thaliana


Overview

The X-ray crystal structure of the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS) from Arabidopsis thaliana has been solved at 2.3 A resolution in complex with a cytidine-5-monophosphate (CMP) molecule. This is the first structure determined of an MCS enzyme from a plant. Major differences between the A. thaliana and bacterial MCS structures are found in the large molecular cavity that forms between subunits and involve residues that are highly conserved among plants. In some bacterial enzymes, the corresponding cavity has been shown to be an isoprenoid diphosphate-like binding pocket, with a proposed feedback-regulatory role. Instead, in the structure from A. thaliana the cavity is unsuited for binding a diphosphate moiety, which suggests a different regulatory mechanism of MCS enzymes between bacteria and plants.

About this Structure

2PMP is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Biosynthesis of isoprenoids in plants: structure of the 2C-methyl-D-erithrytol 2,4-cyclodiphosphate synthase from Arabidopsis thaliana. Comparison with the bacterial enzymes., Calisto BM, Perez-Gil J, Bergua M, Querol-Audi J, Fita I, Imperial S, Protein Sci. 2007 Sep;16(9):2082-8. Epub 2007 Jul 27. PMID:17660251

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