2pnh
From Proteopedia
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| - | [[Image:2pnh.jpg|left|200px]] | + | [[Image:2pnh.jpg|left|200px]] |
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| - | '''Escherichia coli PriB E39A variant''' | + | {{Structure |
| + | |PDB= 2pnh |SIZE=350|CAPTION= <scene name='initialview01'>2pnh</scene>, resolution 2.250Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= priB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Escherichia coli PriB E39A variant''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PNH is a [ | + | 2PNH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNH OCA]. |
==Reference== | ==Reference== | ||
| - | A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:[http:// | + | A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17588514 17588514] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ob fold]] | [[Category: ob fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:15:11 2008'' |
Revision as of 16:15, 20 March 2008
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| , resolution 2.250Å | |||||||
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| Gene: | priB (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Escherichia coli PriB E39A variant
Overview
Collapsed DNA replication forks must be reactivated through origin-independent reloading of the replication machinery (replisome) to ensure complete duplication of cellular genomes. In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome reloading. However, the molecular mechanisms that regulate origin-independent replisome loading are not fully understood. Here, we demonstrate that assembly of primosome protein complexes represents a key regulatory mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are strongly stimulated by single-stranded DNA. Furthermore, the binding site on PriB for single-stranded DNA partially overlaps the binding sites for PriA and DnaT, suggesting a dynamic primosome assembly process in which single-stranded DNA is handed off from one primosome protein to another as a repaired replication fork is reactivated. This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome.
About this Structure
2PNH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:17588514
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