2pnf
From Proteopedia
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| - | [[Image:2pnf.jpg|left|200px]] | + | [[Image:2pnf.jpg|left|200px]] |
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| - | '''Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase''' | + | {{Structure |
| + | |PDB= 2pnf |SIZE=350|CAPTION= <scene name='initialview01'>2pnf</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene> and <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] | ||
| + | |GENE= fabG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PNF is a [ | + | 2PNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNF OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and X-ray diffraction analysis of the beta-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5., Mao Q, Duax WL, Umland TC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):106-9. Epub 2007 Jan 17. PMID:[http:// | + | Crystallization and X-ray diffraction analysis of the beta-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5., Mao Q, Duax WL, Umland TC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):106-9. Epub 2007 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17277451 17277451] |
[[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]] | [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
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[[Category: short chain oxidoreductase]] | [[Category: short chain oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:15:12 2008'' |
Revision as of 16:15, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Ligands: | and | ||||||
| Gene: | fabG (Aquifex aeolicus) | ||||||
| Activity: | [acyl-carrier-protein_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number 1.1.1.100 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Aquifex Aeolicus FabG 3-oxoacyl-(acyl-carrier protein) reductase
Overview
The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 A and the initial phases were determined by molecular replacement. The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier protein reductase. Structure-function studies of this protein are being performed as part of a larger project investigating naturally occurring deviations from highly conserved residues within the short-chain oxidoreductase (SCOR) family.
About this Structure
2PNF is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray diffraction analysis of the beta-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5., Mao Q, Duax WL, Umland TC, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Feb 1;63(Pt, 2):106-9. Epub 2007 Jan 17. PMID:17277451 [[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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