2ppl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ppl.jpg|left|200px]]<br /><applet load="2ppl" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2ppl.jpg|left|200px]]
-
caption="2ppl, resolution 2.20&Aring;" />
+
 
-
'''Human Pancreatic lipase-related protein 1'''<br />
+
{{Structure
 +
|PDB= 2ppl |SIZE=350|CAPTION= <scene name='initialview01'>2ppl</scene>, resolution 2.20&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]
 +
|GENE= PNLIPRP1, PLRP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
}}
 +
 
 +
'''Human Pancreatic lipase-related protein 1'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2PPL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PPL OCA].
+
2PPL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PPL OCA].
==Reference==
==Reference==
-
Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity., Giller T, Buchwald P, Blum-Kaelin D, Hunziker W, J Biol Chem. 1992 Aug 15;267(23):16509-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1379598 1379598]
+
Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity., Giller T, Buchwald P, Blum-Kaelin D, Hunziker W, J Biol Chem. 1992 Aug 15;267(23):16509-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1379598 1379598]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 33: Line 42:
[[Category: structural genomics consortium]]
[[Category: structural genomics consortium]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:54 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:15:58 2008''

Revision as of 16:15, 20 March 2008

Image:2ppl.jpg


PDB ID 2ppl

Drag the structure with the mouse to rotate
, resolution 2.20Å
Ligands: and
Gene: PNLIPRP1, PLRP1 (Homo sapiens)
Activity: Triacylglycerol lipase, with EC number 3.1.1.3
Coordinates: save as pdb, mmCIF, xml



Human Pancreatic lipase-related protein 1


Overview

We have isolated cDNAs coding for two novel human pancreatic lipase (hPL)-related human proteins, referred to as hPL-related proteins 1 and 2 (hPLRP1 and hPLRP2) and for hPL. The two novel proteins show an amino acid sequence identity to hPL of 68 and 65% for hPLRP1 and 2, respectively. All three proteins are secreted into the medium after transfection of COS cells with the corresponding cDNAs. The size of the three expressed proteins is similar and ranges between 45 and 50 kDa. The expressed hPLRP2 shows a lipolytic activity that is, however, in contrast to that of hPL only marginally dependent on the presence of colipase, whereas hPLRP1 shows no activity in this assay. A Northern analysis of normal human pancreas mRNA shows that the expression levels of hPLRP1 and hPLRP2 are about 4-fold and 24-fold lower, respectively, than that of hPL. hPLRP2 is, additionally, most closely related to a lipase reported to be expressed in mouse T-cells. A comparison of the sequences of the three proteins with sequences described as pancreatic lipases of other animal species shows three subfamilies of closer kinship. This suggests that the two novel proteins also exist in other species and that some of the sequences reported to be pancreatic lipase might more likely be the orthologues of hPLRP1 or hPLRP2 in those species.

About this Structure

2PPL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity., Giller T, Buchwald P, Blum-Kaelin D, Hunziker W, J Biol Chem. 1992 Aug 15;267(23):16509-16. PMID:1379598

Page seeded by OCA on Thu Mar 20 18:15:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ppl"
Personal tools