4fof
From Proteopedia
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| - | + | ==Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain== | |
| - | + | <StructureSection load='4fof' size='340' side='right' caption='[[4fof]], [[Resolution|resolution]] 2.42Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4fof]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_bp-1 Thermosynechococcus elongatus bp-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FOF FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=VRB:PHYCOVIOLOBILIN,+BLUE+LIGHT-ABSORBING+FORM'>VRB</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tll0569 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 Thermosynechococcus elongatus BP-1]), HO1 and PcyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fof OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fof RCSB], [http://www.ebi.ac.uk/pdbsum/4fof PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The phytochrome superfamily encompasses a diverse collection of photochromic photoreceptors in plants and microorganisms that employ a covalently linked bilin cradled in a cGMP-phosphodiesterase/adenylyl-cyclase/FhlA (GAF) domain to detect light. Whereas most interconvert between red- and far-red-light-absorbing states, cyanobacteria also express variants called cyanobacteriochromes (CBCRs) that modify bilin absorption to collectively perceive the entire visible spectrum. Here, we present two X-ray crystallographic structures of the GAF domain from the blue/green photochromic CBCR PixJ from Thermosynechococcus elongatus. These structures confirm the hypothesis that CBCRs variably manipulate the chromophore pi-conjugation system through isomerization and a second thioether linkage, in this case involving the bilin C10 carbon and Cys494 within a DXCF sequence characteristic of blue/green CBCRs. Biochemical studies support a mechanism for photoconversion whereby the second linkage ruptures on route to the green-light-absorbing state. Collectively, the TePixJ(GAF) models illustrate the remarkable structural and photochemical versatility among phytochromes and CBCRs in driving light perception. | ||
| - | + | A Photo-Labile Thioether Linkage to Phycoviolobilin Provides the Foundation for the Blue/Green Photocycles in DXCF-Cyanobacteriochromes.,Burgie ES, Walker JM, Phillips GN Jr, Vierstra RD Structure. 2013 Jan 8;21(1):88-97. doi: 10.1016/j.str.2012.11.001. Epub 2012 Dec , 6. PMID:23219880<ref>PMID:23219880</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Chemotaxis protein|Chemotaxis protein]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Theeb]] | ||
[[Category: Thermosynechococcus elongatus bp-1]] | [[Category: Thermosynechococcus elongatus bp-1]] | ||
| - | [[Category: Burgie, E S | + | [[Category: Burgie, E S]] |
| - | [[Category: Phillips, G N | + | [[Category: Phillips, G N]] |
| - | [[Category: Vierstra, R D | + | [[Category: Vierstra, R D]] |
| - | [[Category: Walker, J M | + | [[Category: Walker, J M]] |
[[Category: Blue/green photoreceptor]] | [[Category: Blue/green photoreceptor]] | ||
[[Category: Cyanobacteriochrome]] | [[Category: Cyanobacteriochrome]] | ||
Revision as of 07:45, 15 February 2015
Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain
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