4g6u
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==CdiA-CT/CdiI toxin and immunity complex from Escherichia coli== |
| + | <StructureSection load='4g6u' size='340' side='right' caption='[[4g6u]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4g6u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6U FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g6v|4g6v]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6u RCSB], [http://www.ebi.ac.uk/pdbsum/4g6u PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Contact-dependent growth inhibition (CDI) systems encode polymorphic toxin/immunity proteins that mediate competition between neighboring bacterial cells. We present crystal structures of CDI toxin/immunity complexes from Escherichia coli EC869 and Burkholderia pseudomallei 1026b. Despite sharing little sequence identity, the toxin domains are structurally similar and have homology to endonucleases. The EC869 toxin is a Zn(2+)-dependent DNase capable of completely degrading the genomes of target cells, whereas the Bp1026b toxin cleaves the aminoacyl acceptor stems of tRNA molecules. Each immunity protein binds and inactivates its cognate toxin in a unique manner. The EC869 toxin/immunity complex is stabilized through an unusual beta-augmentation interaction. In contrast, the Bp1026b immunity protein exploits shape and charge complementarity to occlude the toxin active site. These structures represent the initial glimpse into the CDI toxin/immunity network, illustrating how sequence-diverse toxins adopt convergent folds yet retain distinct binding interactions with cognate immunity proteins. Moreover, we present visual demonstration of CDI toxin delivery into a target cell. | ||
| - | + | Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems.,Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21480-5. doi:, 10.1073/pnas.1216238110. Epub 2012 Dec 10. PMID:23236156<ref>PMID:23236156</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Escherichia coli o157:h7]] | [[Category: Escherichia coli o157:h7]] | ||
| - | [[Category: Gerrick, E | + | [[Category: Gerrick, E]] |
| - | [[Category: Goulding, C W | + | [[Category: Goulding, C W]] |
| - | [[Category: Hayes, C S | + | [[Category: Hayes, C S]] |
| - | [[Category: Low, D A | + | [[Category: Low, D A]] |
| - | [[Category: Morse, R P | + | [[Category: Morse, R P]] |
| - | [[Category: Nikolakakis, K | + | [[Category: Nikolakakis, K]] |
| - | [[Category: Willet, J | + | [[Category: Willet, J]] |
[[Category: Beta-augmentation]] | [[Category: Beta-augmentation]] | ||
[[Category: Dnase]] | [[Category: Dnase]] | ||
[[Category: Immunity]] | [[Category: Immunity]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 07:59, 15 February 2015
CdiA-CT/CdiI toxin and immunity complex from Escherichia coli
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