4jax
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of dimeric KlHxk1 in crystal form X== | |
| - | + | <StructureSection load='4jax' size='340' side='right' caption='[[4jax]], [[Resolution|resolution]] 2.26Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4jax]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis_nrrl_y-1140 Kluyveromyces lactis nrrl y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JAX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JAX FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3o08|3o08]], [[3o1b|3o1b]], [[3o1w|3o1w]], [[3o4w|3o4w]], [[3o5b|3o5b]], [[3o6w|3o6w]], [[3o80|3o80]], [[3o8m|3o8m]], [[4jb1|4jb1]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0D11352g, RAG5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Kluyveromyces lactis NRRL Y-1140])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jax OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jax RCSB], [http://www.ebi.ac.uk/pdbsum/4jax PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The bifunctional hexokinase KlHxk1 is a key component of glucose dependent signal transduction in Kluyveromyces lactis. KlHxk1 is phosphorylated in vivo and undergoes ATP-dependent autophosphorylation-inactivation in vitro. This study identifies serine-15 as the site of in vivo phosphorylation and serine-157 as the autophosphorylation-inactivation site. X-ray crystallography of the in vivo phosphorylated enzyme indicates the existence of a ring-shaped symmetrical homodimer carrying two phosphoserine-15 residues. In contrast, small-angle X-ray scattering and equilibrium sedimentation analyses reveal the existence of monomeric phosphoserine-15 KlHxk1 in solution. While phosphorylation at serine-15 and concomitant homodimer dissociation are likely to be involved in glucose signalling, mechanism and putative physiological significance of KlHxk1 inactivation by autophosphorylation at serine-157 remain to be established. | ||
| - | + | In vivo phosphorylation and in vitro autophosphorylation-inactivation of Kluyveromyces lactis hexokinase KlHxk1.,Kettner K, Kuettner EB, Otto A, Lilie H, Golbik RP, Strater N, Kriegel TM Biochem Biophys Res Commun. 2013 Apr 10. pii: S0006-291X(13)00600-1. doi:, 10.1016/j.bbrc.2013.03.121. PMID:23583397<ref>PMID:23583397</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Hexokinase|Hexokinase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Hexokinase]] | [[Category: Hexokinase]] | ||
[[Category: Kluyveromyces lactis nrrl y-1140]] | [[Category: Kluyveromyces lactis nrrl y-1140]] | ||
| - | [[Category: Golbik, R P | + | [[Category: Golbik, R P]] |
| - | [[Category: Kettner, K | + | [[Category: Kettner, K]] |
| - | [[Category: Kriegel, T M | + | [[Category: Kriegel, T M]] |
| - | [[Category: Kuettner, E B | + | [[Category: Kuettner, E B]] |
| - | [[Category: Lilie, H | + | [[Category: Lilie, H]] |
| - | [[Category: Otto, A | + | [[Category: Otto, A]] |
| - | [[Category: Strater, N | + | [[Category: Strater, N]] |
[[Category: Atp binding]] | [[Category: Atp binding]] | ||
| - | [[Category: Hexokinase]] | ||
[[Category: Mig1 binding]] | [[Category: Mig1 binding]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
Revision as of 09:16, 15 February 2015
Crystal structure of dimeric KlHxk1 in crystal form X
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