2pxj

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[[Image:2pxj.jpg|left|200px]]<br /><applet load="2pxj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2pxj.jpg|left|200px]]
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caption="2pxj, resolution 2.00&Aring;" />
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'''The complex structure of JMJD2A and monomethylated H3K36 peptide'''<br />
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{{Structure
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|PDB= 2pxj |SIZE=350|CAPTION= <scene name='initialview01'>2pxj</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> and <scene name='pdbligand=OGA:N-OXALYOLGLYCINE'>OGA</scene>
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|ACTIVITY=
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|GENE= JMJD2A, JHDM3A, JMJD2, KIAA0677 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''The complex structure of JMJD2A and monomethylated H3K36 peptide'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2PXJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=FE2:'>FE2</scene> and <scene name='pdbligand=OGA:'>OGA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PXJ OCA].
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2PXJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PXJ OCA].
==Reference==
==Reference==
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Structural basis of the recognition of a methylated histone tail by JMJD2A., Chen Z, Zang J, Kappler J, Hong X, Crawford F, Wang Q, Lan F, Jiang C, Whetstine J, Dai S, Hansen K, Shi Y, Zhang G, Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10818-23. Epub 2007 Jun 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17567753 17567753]
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Structural basis of the recognition of a methylated histone tail by JMJD2A., Chen Z, Zang J, Kappler J, Hong X, Crawford F, Wang Q, Lan F, Jiang C, Whetstine J, Dai S, Hansen K, Shi Y, Zhang G, Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10818-23. Epub 2007 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17567753 17567753]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: jmjd2a; histone demethylase; jmjc; h3k36]]
[[Category: jmjd2a; histone demethylase; jmjc; h3k36]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:18:40 2008''

Revision as of 16:18, 20 March 2008

Image:2pxj.jpg


PDB ID 2pxj

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: , and
Gene: JMJD2A, JHDM3A, JMJD2, KIAA0677 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



The complex structure of JMJD2A and monomethylated H3K36 peptide


Overview

The Jumonji C domain is a catalytic motif that mediates histone lysine demethylation. The Jumonji C-containing oxygenase JMJD2A specifically demethylates tri- and dimethylated lysine-9 and lysine-36 of histone 3 (H3K9/36 me3/2). Here we present structures of the JMJD2A catalytic core complexed with methylated H3K36 peptide substrates in the presence of Fe(II) and N-oxalylglycine. We found that the interaction between JMJD2A and peptides largely involves the main chains of the enzyme and the peptide. The peptide-binding specificity is primarily determined by the primary structure of the peptide, which explains the specificity of JMJD2A for methylated H3K9 and H3K36 instead of other methylated residues such as H3K27. The specificity for a particular methyl group, however, is affected by multiple factors, such as space and the electrostatic environment in the catalytic center of the enzyme. These results provide insights into the mechanisms and specificity of histone demethylation.

About this Structure

2PXJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of the recognition of a methylated histone tail by JMJD2A., Chen Z, Zang J, Kappler J, Hong X, Crawford F, Wang Q, Lan F, Jiang C, Whetstine J, Dai S, Hansen K, Shi Y, Zhang G, Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10818-23. Epub 2007 Jun 13. PMID:17567753

Page seeded by OCA on Thu Mar 20 18:18:40 2008

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