2pzj
From Proteopedia
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| - | [[Image:2pzj.jpg|left|200px]] | + | [[Image:2pzj.jpg|left|200px]] |
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| - | '''Crystal structure of the Bordetella bronchiseptica enzyme WbmF in complex with NAD+''' | + | {{Structure |
| + | |PDB= 2pzj |SIZE=350|CAPTION= <scene name='initialview01'>2pzj</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= BbLPS1.16, wbmF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=518 Bordetella bronchiseptica]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Bordetella bronchiseptica enzyme WbmF in complex with NAD+''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2PZJ is a [ | + | 2PZJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bordetella_bronchiseptica Bordetella bronchiseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZJ OCA]. |
==Reference== | ==Reference== | ||
| - | Predicting protein function from structure--the roles of short-chain dehydrogenase/reductase enzymes in Bordetella O-antigen biosynthesis., King JD, Harmer NJ, Preston A, Palmer CM, Rejzek M, Field RA, Blundell TL, Maskell DJ, J Mol Biol. 2007 Nov 30;374(3):749-63. Epub 2007 Sep 26. PMID:[http:// | + | Predicting protein function from structure--the roles of short-chain dehydrogenase/reductase enzymes in Bordetella O-antigen biosynthesis., King JD, Harmer NJ, Preston A, Palmer CM, Rejzek M, Field RA, Blundell TL, Maskell DJ, J Mol Biol. 2007 Nov 30;374(3):749-63. Epub 2007 Sep 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17950751 17950751] |
[[Category: Bordetella bronchiseptica]] | [[Category: Bordetella bronchiseptica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:19:54 2008'' |
Revision as of 16:19, 20 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Gene: | BbLPS1.16, wbmF (Bordetella bronchiseptica) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Bordetella bronchiseptica enzyme WbmF in complex with NAD+
Overview
The pathogenic bacteria Bordetella parapertussis and Bordetella bronchiseptica express a lipopolysaccharide O antigen containing a polymer of 2,3-diacetamido-2,3-dideoxy-l-galacturonic acid. The O-antigen cluster contains three neighbouring genes that encode proteins belonging to the short-chain dehydrogenase/reductase (SDR) family, wbmF, wbmG and wbmH, and we aimed to elucidate their individual functions. Mutation and complementation implicate each gene in O-antigen expression but, as their putative sugar nucleotide substrates are not currently available, biochemical characterisation of WbmF, WbmG and WbmH is impractical at the present time. SDR family members catalyse a wide range of chemical reactions including oxidation, reduction and epimerisation. Because they typically share low sequence conservation, however, catalytic function cannot be predicted from sequence analysis alone. In this context, structural characterisation of the native proteins, co-crystals and small-molecule soaks enables differentiation of the functions of WbmF, WbmG and WbmH. These proteins exhibit typical SDR architecture and coordinate NAD. In the substrate-binding domain, all three enzymes bind uridyl nucleotides. WbmG contains a typical SDR catalytic TYK triad, which is required for oxidoreductase function, but the active site is devoid of additional acid-base functionality. Similarly, WbmH possesses a TYK triad, but an otherwise feature-poor active site. Consequently, 3,5-epimerase function can probably be ruled out for these enzymes. The WbmF active site contains conserved 3,5-epimerase features, namely, a positionally conserved cysteine (Cys133) and basic side chain (His90 or Asn213), but lacks the serine/threonine component of the SDR triad and therefore may not act as an oxidoreductase. The data suggest a pathway for synthesis of the O-antigen precursor UDP-2,3-diacetamido-2,3-dideoxy-l-galacturonic acid and illustrate the usefulness of structural data in predicting protein function.
About this Structure
2PZJ is a Single protein structure of sequence from Bordetella bronchiseptica. Full crystallographic information is available from OCA.
Reference
Predicting protein function from structure--the roles of short-chain dehydrogenase/reductase enzymes in Bordetella O-antigen biosynthesis., King JD, Harmer NJ, Preston A, Palmer CM, Rejzek M, Field RA, Blundell TL, Maskell DJ, J Mol Biol. 2007 Nov 30;374(3):749-63. Epub 2007 Sep 26. PMID:17950751
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