2q0o
From Proteopedia
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| - | [[Image:2q0o.jpg|left|200px]] | + | [[Image:2q0o.jpg|left|200px]] |
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| - | '''Crystal structure of an anti-activation complex in bacterial quorum sensing''' | + | {{Structure |
| + | |PDB= 2q0o |SIZE=350|CAPTION= <scene name='initialview01'>2q0o</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=LAE:3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE'>LAE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= traR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391 Rhizobium sp.]), traM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391 Rhizobium sp.]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of an anti-activation complex in bacterial quorum sensing''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2Q0O is a [ | + | 2Q0O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhizobium_sp. Rhizobium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q0O OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for antiactivation in bacterial quorum sensing., Chen G, Jeffrey PD, Fuqua C, Shi Y, Chen L, Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16474-9. Epub 2007 Oct 5. PMID:[http:// | + | Structural basis for antiactivation in bacterial quorum sensing., Chen G, Jeffrey PD, Fuqua C, Shi Y, Chen L, Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16474-9. Epub 2007 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17921255 17921255] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rhizobium sp.]] | [[Category: Rhizobium sp.]] | ||
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[[Category: two-helix coiled coil]] | [[Category: two-helix coiled coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:20:18 2008'' |
Revision as of 16:20, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Gene: | traR (Rhizobium sp.), traM (Rhizobium sp.) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an anti-activation complex in bacterial quorum sensing
Overview
Bacteria can communicate via diffusible signal molecules they generate and release to coordinate their behavior in response to the environment. Signal molecule concentration is often proportional to bacterial population density, and when this reaches a critical concentration, reflecting a bacterial quorum, specific behaviors including virulence, symbiosis, and horizontal gene transfer are activated. Quorum-sensing regulation in many Gram-negative bacteria involves acylated homoserine lactone signals that are perceived through binding to LuxR-type, acylated-homoserine-lactone-responsive transcription factors. Bacteria of the rhizobial group employ the LuxR-type transcriptional activator TraR in quorum sensing, and its activity is further regulated through interactions with the TraM antiactivator. In this study, we have crystallographically determined the 3D structure of the TraR-TraM antiactivation complex from Rhizobium sp. strain NGR234. Unexpectedly, the antiactivator TraM binds to TraR at a site distinct from its DNA-binding motif and induces an allosteric conformational change in the protein, thereby preventing DNA binding. Structural analysis reveals a highly conserved TraR-TraM interface and suggests a mechanism for antiactivation complex formation. This structure may inform alternative strategies to control quorum-sensing-regulated microbial activity including amelioration of infectious disease and antibiotic resistance. In addition, the structural basis of antiactivation presents a regulatory interaction that provides general insights relevant to the field of transcription regulation and signal transduction.
About this Structure
2Q0O is a Protein complex structure of sequences from Rhizobium sp.. Full crystallographic information is available from OCA.
Reference
Structural basis for antiactivation in bacterial quorum sensing., Chen G, Jeffrey PD, Fuqua C, Shi Y, Chen L, Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16474-9. Epub 2007 Oct 5. PMID:17921255
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