4wqd
From Proteopedia
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| - | ''' | + | ==Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum - K208G mutant== |
| + | <StructureSection load='4wqd' size='340' side='right' caption='[[4wqd]], [[Resolution|resolution]] 1.22Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wqd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WQD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WQD FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GAI:GUANIDINE'>GAI</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wq7|4wq7]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiosulfate_dehydrogenase Thiosulfate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.2.2 1.8.2.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wqd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wqd RCSB], [http://www.ebi.ac.uk/pdbsum/4wqd PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TSDA_ALLVD TSDA_ALLVD]] Catalyzes the oxidation of 2 molecules of thiosulfate to tetrathionate.<ref>PMID:16995898</ref> <ref>PMID:22779704</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ability to perform the very simple oxidation of two molecules of thiosulfate to tetrathionate is widespread among prokaryotes. Despite the prevalent occurrence of tetrathionate formation and its well documented significance within the sulfur cycle, little is known about the enzymes that catalyze the oxidative condensation of two thiosulfate anions. To fill this gap, the thiosulfate dehydrogenase (TsdA) enzyme from the purple sulfur bacterium Allochromatium vinosum was recombinantly expressed in Escherichia coli, purified and crystallized, and a crystallographic data set was collected. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 79.2, b = 69.9, c = 57.9 A, beta = 129.3 degrees , contained one monomer per asymmetric unit and diffracted to a resolution of 1.98 A. | ||
| - | + | Production, crystallization and preliminary crystallographic analysis of Allochromatium vinosum thiosulfate dehydrogenase TsdA, an unusual acidophilic c-type cytochrome.,Brito JA, Gutierres A, Denkmann K, Dahl C, Archer M Acta Crystallogr F Struct Biol Commun. 2014 Oct;70(Pt 10):1424-7. doi:, 10.1107/S2053230X14019384. Epub 2014 Sep 25. PMID:25286955<ref>PMID:25286955</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Thiosulfate dehydrogenase]] | ||
| + | [[Category: Archer, M]] | ||
| + | [[Category: Brito, J A]] | ||
| + | [[Category: Dahl, C]] | ||
[[Category: Denkmann, K]] | [[Category: Denkmann, K]] | ||
| - | [[Category: | + | [[Category: Pereira, I A.C]] |
| - | [[Category: | + | [[Category: C-type cytochrome]] |
| - | [[Category: | + | [[Category: Oxidoreductase]] |
| + | [[Category: Tetrathionate]] | ||
| + | [[Category: Tsda]] | ||
Revision as of 12:57, 18 February 2015
Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum - K208G mutant
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