2q3x

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[[Image:2q3x.jpg|left|200px]]<br /><applet load="2q3x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2q3x.jpg|left|200px]]
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caption="2q3x, resolution 1.73&Aring;" />
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'''The RIM1alpha C2B domain'''<br />
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{{Structure
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|PDB= 2q3x |SIZE=350|CAPTION= <scene name='initialview01'>2q3x</scene>, resolution 1.73&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE= Rims1, Rim1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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}}
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'''The RIM1alpha C2B domain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2Q3X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q3X OCA].
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2Q3X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q3X OCA].
==Reference==
==Reference==
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Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution., Guan R, Dai H, Tomchick DR, Dulubova I, Machius M, Sudhof TC, Rizo J, Biochemistry. 2007 Aug 7;46(31):8988-98. Epub 2007 Jul 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17630786 17630786]
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Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution., Guan R, Dai H, Tomchick DR, Dulubova I, Machius M, Sudhof TC, Rizo J, Biochemistry. 2007 Aug 7;46(31):8988-98. Epub 2007 Jul 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17630786 17630786]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transport protein]]
[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:35:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:21:18 2008''

Revision as of 16:21, 20 March 2008

Image:2q3x.jpg


PDB ID 2q3x

Drag the structure with the mouse to rotate
, resolution 1.73Å
Ligands: , and
Gene: Rims1, Rim1 (Rattus norvegicus)
Coordinates: save as pdb, mmCIF, xml



The RIM1alpha C2B domain


Overview

RIM proteins play critical roles in synaptic vesicle priming and diverse forms of presynaptic plasticity. The C-terminal C2B domain is the only module that is common to all RIMs but is only distantly related to well-studied C2 domains, and its three-dimensional structure and interactions have not been characterized in detail. Using NMR spectroscopy, we now show that N- and C-terminal extensions beyond the predicted C2B domain core sequence are necessary to form a folded, stable RIM1alpha C2B domain. We also find that the isolated RIM1alpha C2B domain is not sufficient for previously described protein-protein interactions involving the RIM1alpha C-terminus, suggesting that additional sequences adjacent to the C2B domain might be required for these interactions. However, analytical ultracentrifugation shows that the RIM1alpha C2B domain forms weak dimers in solution. The crystal structure of the RIM1alpha C2B domain dimer at 1.7 A resolution reveals that it forms a beta-sandwich characteristic of C2 domains and that the unique N- and C-terminal extensions form a small subdomain that packs against the beta-sandwich and mediates dimerization. Our results provide a structural basis to understand the function of RIM C2B domains and suggest that dimerization may be a crucial aspect of RIM function.

About this Structure

2Q3X is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution., Guan R, Dai H, Tomchick DR, Dulubova I, Machius M, Sudhof TC, Rizo J, Biochemistry. 2007 Aug 7;46(31):8988-98. Epub 2007 Jul 14. PMID:17630786

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