4l6u
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6u RCSB], [http://www.ebi.ac.uk/pdbsum/4l6u PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l6u RCSB], [http://www.ebi.ac.uk/pdbsum/4l6u PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A multi-subunit ribonucleoprotein complex termed the Cmr RNA-silencing complex recognizes and destroys viral RNA in the CRISPR-mediated immune defence mechanism in many prokaryotes using an as yet unclear mechanism. In Archaeoglobus fulgidus, this complex consists of six subunits, Cmr1-Cmr6. Here, the crystal structure of Cmr1 from A. fulgidus is reported, revealing that the protein is composed of two tightly associated ferredoxin-like domains. The domain located at the N-terminus is structurally most similar to the N-terminal ferredoxin-like domain of the CRISPR RNA-processing enzyme Cas6 from Pyrococcus furiosus. An ensuing mutational analysis identified a highly conserved basic surface patch that binds single-stranded nucleic acids specifically, including the mature CRISPR RNA, but in a sequence-independent manner. In addition, this subunit was found to cleave single-stranded RNA. Together, these studies elucidate the structure and the catalytic activity of the Cmr1 subunit. | ||
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| + | Crystal structure and CRISPR RNA-binding site of the Cmr1 subunit of the Cmr interference complex.,Sun J, Jeon JH, Shin M, Shin HC, Oh BH, Kim JS Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):535-43. doi:, 10.1107/S1399004713030290. Epub 2014 Jan 31. PMID:24531487<ref>PMID:24531487</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:12, 25 February 2015
Crystal structure of AF1868: Cmr1 subunit of the Cmr RNA silencing complex
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Categories: Arcfu | Jeon, J H | Kim, J S | Oh, B H | Shin, H C | Shin, M | Sun, J | Ferredoxin | Unknown function
