2q8a
From Proteopedia
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| - | [[Image:2q8a.jpg|left|200px]] | + | [[Image:2q8a.jpg|left|200px]] |
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| - | '''Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody''' | + | {{Structure |
| + | |PDB= 2q8a |SIZE=350|CAPTION= <scene name='initialview01'>2q8a</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= AMA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2Q8A is a [ | + | 2Q8A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8A OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody., Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Anders RF, Foley M, Batchelor AH, PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:[http:// | + | Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody., Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Anders RF, Foley M, Batchelor AH, PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17907804 17907804] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
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[[Category: immune system]] | [[Category: immune system]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:22:55 2008'' |
Revision as of 16:22, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | AMA1 (Plasmodium falciparum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody
Overview
Identifying functionally critical regions of the malaria antigen AMA1 (apical membrane antigen 1) is necessary to understand the significance of the polymorphisms within this antigen for vaccine development. The crystal structure of AMA1 in complex with the Fab fragment of inhibitory monoclonal antibody 1F9 reveals that 1F9 binds to the AMA1 solvent-exposed hydrophobic trough, confirming its importance. 1F9 uses the heavy and light chain complementarity-determining regions (CDRs) to wrap around the polymorphic loops adjacent to the trough, but uses a ridge of framework residues to bind to the hydrophobic trough. The resulting 1F9-AMA1-combined buried surface of 2,470 A(2) is considerably larger than previously reported Fab-antigen interfaces. Mutations of polymorphic AMA1 residues within the 1F9 epitope disrupt 1F9 binding and dramatically reduce the binding of affinity-purified human antibodies. Moreover, 1F9 binding to AMA1 is competed by naturally acquired human antibodies, confirming that the 1F9 epitope is a frequent target of immunological attack.
About this Structure
2Q8A is a Single protein structure of sequence from Mus musculus and Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody., Coley AM, Gupta A, Murphy VJ, Bai T, Kim H, Anders RF, Foley M, Batchelor AH, PLoS Pathog. 2007 Sep 7;3(9):1308-19. PMID:17907804
Page seeded by OCA on Thu Mar 20 18:22:55 2008
