2q8y

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[[Image:2q8y.jpg|left|200px]]<br /><applet load="2q8y" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2q8y.jpg|left|200px]]
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caption="2q8y, resolution 2.00&Aring;" />
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'''Structural insight into the enzymatic mechanism of the phophothreonine lyase'''<br />
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{{Structure
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|PDB= 2q8y |SIZE=350|CAPTION= <scene name='initialview01'>2q8y</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2. 4.2.]
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|GENE= mkaD, spvC, vsdD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=592 Salmonella enteritidis])
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}}
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'''Structural insight into the enzymatic mechanism of the phophothreonine lyase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2Q8Y is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_enteritidis Salmonella enteritidis]. Active as [http://en.wikipedia.org/wiki/Lyase Lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2. 4.2.] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8Y OCA].
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2Q8Y is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_enteritidis Salmonella enteritidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q8Y OCA].
==Reference==
==Reference==
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Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase., Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F, Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18060821 18060821]
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Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase., Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F, Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18060821 18060821]
[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: lyase/transferase complex]]
[[Category: lyase/transferase complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:37:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:23:13 2008''

Revision as of 16:23, 20 March 2008


PDB ID 2q8y

Drag the structure with the mouse to rotate
, resolution 2.00Å
Gene: mkaD, spvC, vsdD (Salmonella enteritidis)
Activity: Lyase, with EC number 4.2.
Coordinates: save as pdb, mmCIF, xml



Structural insight into the enzymatic mechanism of the phophothreonine lyase


Overview

The OspF family of phosphothreonine lyase, including SpvC from Salmonella, irreversibly inactivates the dual-phosphorylated host MAPKs (pT-X-pY) through beta elimination. We determined crystal structures of SpvC and its complex with a phosphopeptide substrate. SpvC adopts a unique fold of alpha/beta type. The disordered N terminus harbors a canonical D motif for MAPK substrate docking. The enzyme-substrate complex structure indicates that recognition of the phosphotyrosine followed by insertion of the threonine phosphate into an arginine pocket places the phosphothreonine into the enzyme active site. This requires the conformational flexibility of pT-X-pY, which suggests that p38 (pT-G-pY) is likely the preferred physiological substrate. Structure-based biochemical and enzymatic analysis allows us to propose a general acid/base mechanism for beta elimination reaction catalyzed by the phosphothreonine lyase. The mechanism described here provides a structural understanding of MAPK inactivation by a family of pathogenic effectors conserved in plant and animal systems and may also open a new route for biological catalysis.

About this Structure

2Q8Y is a Protein complex structure of sequences from Salmonella enteritidis. Full crystallographic information is available from OCA.

Reference

Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase., Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F, Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:18060821

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