User:Shai Biran/Practice Proteopedia tutorial shai3

From Proteopedia

< User:Shai Biran(Difference between revisions)
Jump to: navigation, search
Current revision (13:09, 25 February 2015) (edit) (undo)
 
Line 18: Line 18:
== Introduction ==
== Introduction ==
-
Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
+
Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry 1ema), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
== Exploring the Structure ==
== Exploring the Structure ==
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The
-
<scene name='Sandbox_4/Green_fp/1'>chromophore</scene>, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>
+
chromophore, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>

Current revision

Contents

Adding Section Headings

  1. In your assigned Sandbox page, now displaying your new text, click “edit this page”.
  2. We want to make “Introduction” and “Exploring the Structure” as a section headings.
  3. Type the text “Introduction” just before the words: “Green fluorescent protein (GFP),”and select the button from the toolbar above the text-editing area with a big “A”.
  4. The result should be two equals signs on either side of the words “Introduction”. It should look like this: ==Introduction==.
  5. In a similar way, add a heading: “Exploring the Structure”, just before “GFP is a beta barrel protein with 11 beta sheets…”
  6. Delete the headings: Function, Disease, Relevance, Structural highlights
  7. Save the page using the “Save page” button.

Your page should look like this: ↓↓↓↓↓↓↓↓↓↓↓↓

Your Heading Here (maybe something like 'Structure')

Caption for this structure

Drag the structure with the mouse to rotate

Next

In the next page we will learn about Bold and Interwiki links

Previous page (3)

Next Page (5)


References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
  3. ↑ Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

Proteopedia Page Contributors and Editors (what is this?)

Shai Biran

Retrieved from "http://52.214.119.220/wiki/index.php/User:Shai_Biran/Practice_Proteopedia_tutorial_shai3"
Personal tools