2qca

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[[Image:2qca.jpg|left|200px]]<br /><applet load="2qca" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2qca.jpg|left|200px]]
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caption="2qca, resolution 1.33&Aring;" />
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'''A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate'''<br />
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{{Structure
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|PDB= 2qca |SIZE=350|CAPTION= <scene name='initialview01'>2qca</scene>, resolution 1.33&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=DGP:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DGP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
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|GENE=
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}}
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'''A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2QCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=DGP:'>DGP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCA OCA].
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2QCA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCA OCA].
==Reference==
==Reference==
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A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate., Larson SB, Day JS, Cudney R, McPherson A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):728-33. Epub 2007 Aug 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17768339 17768339]
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A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate., Larson SB, Day JS, Cudney R, McPherson A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):728-33. Epub 2007 Aug 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17768339 17768339]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Pancreatic ribonuclease]]
[[Category: Pancreatic ribonuclease]]
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[[Category: psi-2]]
[[Category: psi-2]]
[[Category: ribonuclease]]
[[Category: ribonuclease]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:38:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:24:17 2008''

Revision as of 16:24, 20 March 2008

Image:2qca.jpg


PDB ID 2qca

Drag the structure with the mouse to rotate
, resolution 1.33Å
Ligands:
Activity: Pancreatic ribonuclease, with EC number 3.1.27.5
Coordinates: save as pdb, mmCIF, xml



A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate


Overview

The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at room temperature in a previously unreported unit cell belonging to space group P3(1). There are two molecules of nucleotide per enzyme molecule, one of which lies in the active-site cleft in the productive binding mode, whilst the guanine base of the other dGMP occupies the pyrimidine-specific binding site in a nonproductive mode such that it forms hydrogen bonds to the phosphate group of the first dGMP. This is the first RNase A structure containing a guanine base in the B2 binding site. Each dGMP molecule is involved in intermolecular interactions with adjacent RNase A molecules in the lattice and the two nucleotides appear to direct the formation of the crystal lattice. Because GMP may be produced during degradation of RNA, this association could represent an inhibitor complex and thereby affect the observed enzyme kinetics.

About this Structure

2QCA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate., Larson SB, Day JS, Cudney R, McPherson A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):728-33. Epub 2007 Aug 31. PMID:17768339

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