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2qcx
From Proteopedia
(Difference between revisions)
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| - | [[Image:2qcx.jpg|left|200px]] | + | [[Image:2qcx.jpg|left|200px]] |
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| - | '''Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine''' | + | {{Structure |
| + | |PDB= 2qcx |SIZE=350|CAPTION= <scene name='initialview01'>2qcx</scene>, resolution 2.200Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Pf1+Binding+Site+For+Residue+A+300'>AC1</scene> and <scene name='pdbsite=AC2:Pf1+Binding+Site+For+Residue+B+300'>AC2</scene> | ||
| + | |LIGAND= <scene name='pdbligand=PF1:N-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-N-(2-HYDROXYETHYL)FORMAMIDE'>PF1</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] | ||
| + | |GENE= tenA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QCX is a [ | + | 2QCX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QCX OCA]. |
==Reference== | ==Reference== | ||
| - | Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:[http:// | + | Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18054064 18054064] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: up-down bundle]] | [[Category: up-down bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:24:31 2008'' |
Revision as of 16:24, 20 March 2008
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| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | |||||||
| Gene: | tenA (Bacillus subtilis) | ||||||
| Activity: | Thiaminase, with EC number 3.5.99.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine
Overview
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.
About this Structure
2QCX is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:18054064
Page seeded by OCA on Thu Mar 20 18:24:31 2008
