2qku
From Proteopedia
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| - | [[Image:2qku.jpg|left|200px]] | + | [[Image:2qku.jpg|left|200px]] |
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| - | '''The 5th PDZ Domain of InaD in 10mM DTT''' | + | {{Structure |
| + | |PDB= 2qku |SIZE=350|CAPTION= <scene name='initialview01'>2qku</scene>, resolution 2.200Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= inaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''The 5th PDZ Domain of InaD in 10mM DTT''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QKU is a [ | + | 2QKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QKU OCA]. |
==Reference== | ==Reference== | ||
| - | Dynamic scaffolding in a g protein-coupled signaling system., Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R, Cell. 2007 Oct 5;131(1):80-92. PMID:[http:// | + | Dynamic scaffolding in a g protein-coupled signaling system., Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R, Cell. 2007 Oct 5;131(1):80-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17923089 17923089] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vision]] | [[Category: vision]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:26:54 2008'' |
Revision as of 16:26, 20 March 2008
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| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | inaD (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The 5th PDZ Domain of InaD in 10mM DTT
Overview
The INAD scaffold organizes a multiprotein complex that is essential for proper visual signaling in Drosophila photoreceptor cells. Here we show that one of the INAD PDZ domains (PDZ5) exists in a redox-dependent equilibrium between two conformations--a reduced form that is similar to the structure of other PDZ domains, and an oxidized form in which the ligand-binding site is distorted through formation of a strong intramolecular disulfide bond. We demonstrate transient light-dependent formation of this disulfide bond in vivo and find that transgenic flies expressing a mutant INAD in which PDZ5 is locked in the reduced state display severe defects in termination of visual responses and visually mediated reflex behavior. These studies demonstrate a conformational switch mechanism for PDZ domain function and suggest that INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction at the millisecond timescale through cycles of conformational change.
About this Structure
2QKU is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Dynamic scaffolding in a g protein-coupled signaling system., Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R, Cell. 2007 Oct 5;131(1):80-92. PMID:17923089
Page seeded by OCA on Thu Mar 20 18:26:54 2008
