2qrc
From Proteopedia
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| - | [[Image:2qrc.jpg|left|200px]] | + | [[Image:2qrc.jpg|left|200px]] |
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| - | '''Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP''' | + | {{Structure |
| + | |PDB= 2qrc |SIZE=350|CAPTION= <scene name='initialview01'>2qrc</scene>, resolution 2.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | ||
| + | |GENE= ssp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2QRC is a [ | + | 2QRC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRC OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insight into AMPK regulation: ADP comes into play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:[http:// | + | Structural insight into AMPK regulation: ADP comes into play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937917 17937917] |
[[Category: Non-specific serine/threonine protein kinase]] | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:28:49 2008'' |
Revision as of 16:28, 20 March 2008
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| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | ssp2 (Schizosaccharomyces pombe) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP
Overview
The AMP-activated protein kinase (AMPK), a sensor of cellular energy status found in all eukaryotes, responds to changes in intracellular adenosine nucleotide levels resulting from metabolic stresses. Here we describe crystal structures of a heterotrimeric regulatory core fragment from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR phosphate, or ZMP), a well-characterized AMPK activator. Prior crystallographic studies had revealed a single site in the gamma subunit that binds either ATP or AMP within Bateman domain B. Here we show that ZMP binds at this site, mimicking the binding of AMP. An analogous site in Bateman domain A selectively accommodates ADP, which binds in a distinct manner that also involves direct ligation to elements from the beta subunit. These observations suggest a possible role for ADP in regulating AMPK response to changes in cellular energy status.
About this Structure
2QRC is a Protein complex structure of sequences from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Structural insight into AMPK regulation: ADP comes into play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:17937917
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