This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Sandbox Reserved 433
From Proteopedia
(Difference between revisions)
| Line 10: | Line 10: | ||
==Introduction== | ==Introduction== | ||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. | ||
| - | |||
| - | <Structure load='1q3d' size='300' frame='true' align='right' caption='3P8Z, I am inserting the caption here.' scene='Insert optional scene name here' /> | ||
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. | ||
| - | |||
| Line 35: | Line 31: | ||
<scene name='48/483890/1q3d/1'>1q3d structure</scene> | <scene name='48/483890/1q3d/1'>1q3d structure</scene> | ||
==Binding Interactions== | ==Binding Interactions== | ||
| + | |||
1)Folding of protein | 1)Folding of protein | ||
a) location and description of position of alpha and beta sheets (Show in separate color on green screen) | a) location and description of position of alpha and beta sheets (Show in separate color on green screen) | ||
| Line 50: | Line 47: | ||
2. Difference that can be observed from GSK-3 beta complexes with Staurosporine and other inhibitors (AMP-PNP, indirubin-3'-monoxime) | 2. Difference that can be observed from GSK-3 beta complexes with Staurosporine and other inhibitors (AMP-PNP, indirubin-3'-monoxime) | ||
| + | <scene name='48/483890/1q3d/1'>1q3d structure</scene> | ||
| + | |||
==Quiz Question 1== | ==Quiz Question 1== | ||
| - | |||
| - | |||
Which isoform of GSK-3 would work best for ___ function in ___ pathway? | Which isoform of GSK-3 would work best for ___ function in ___ pathway? | ||
| - | |||
| - | What kind of bond would GSK-3B form with a staurosporine complex? Picture is shown below. | ||
| - | [[Media:http://upload.wikimedia.org/wikipedia/commons/3/34/Staurosporine1.png]] | ||
| - | |||
| - | Excerpt from GSK-3: Functional Insights from Cell Biology and Animal Models: | ||
| - | "In certain cell types of the brain, alternative splicing between exon 8 and 9 of GSK-3β leads to the generation of an additional “long” form containing a 13 amino acid insert within the catalytic domain (GSK-3β2; see Figure Figure1).1). This insert is located between residues 303 and 304 of GSK-3β, and is flanked by two proximal α-helices of kinase subdomains X and XI (Hanks and Hunter, 1995; Mukai et al., 2002). This alternatively spliced isoform of GSK-3β in rodents (Mukai et al., 2002; Yao et al., 2002) and in human (Lau et al., 1999; Schaffer et al., 2003; Kwok et al., 2005) has been implicated in neuronal-specific functions. The short form of GSK-3β is ubiquitously expressed in the body, including the developing and adult nervous system (Takahashi et al., 1994; Leroy and Brion, 1999). By contrast, GSK-3β2 is predominantly expressed in the neural tissues, with highest levels in the developing brain and persistence into adulthood (Mukai et al., 2002; Wood-Kaczmar et al., 2009)." | ||
| - | |||
| - | "From the crystal structure, it has been proposed that unphosphorylated Tyr276/Tyr216 act to block the access of primed substrates (as discussed below). Indeed, the structure of phosphorylated GSK-3β (Bax et al., 2001) shows that phosphorylated Tyr216 undergoes a conformational change that allows substrates to bind the enzyme" | ||
==Quiz Question 2== | ==Quiz Question 2== | ||
Revision as of 22:55, 12 March 2015
| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
| |||||||||||
