2qvw

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[[Image:2qvw.jpg|left|200px]]<br /><applet load="2qvw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2qvw.jpg|left|200px]]
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caption="2qvw, resolution 3.000&Aring;" />
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'''Structure of Giardia Dicer refined against twinned data'''<br />
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{{Structure
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|PDB= 2qvw |SIZE=350|CAPTION= <scene name='initialview01'>2qvw</scene>, resolution 3.000&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3]
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|GENE=
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}}
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'''Structure of Giardia Dicer refined against twinned data'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2QVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA].
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2QVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVW OCA].
==Reference==
==Reference==
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17704568 17704568]
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An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17704568 17704568]
[[Category: Giardia intestinalis]]
[[Category: Giardia intestinalis]]
[[Category: Ribonuclease III]]
[[Category: Ribonuclease III]]
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[[Category: rnai]]
[[Category: rnai]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:42:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:30:08 2008''

Revision as of 16:30, 20 March 2008


PDB ID 2qvw

Drag the structure with the mouse to rotate
, resolution 3.000Å
Ligands:
Activity: Ribonuclease III, with EC number 3.1.26.3
Coordinates: save as pdb, mmCIF, xml



Structure of Giardia Dicer refined against twinned data


Overview

The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.

About this Structure

2QVW is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.

Reference

An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568

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