2qzv
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2qzv.jpg|left|200px]] | + | [[Image:2qzv.jpg|left|200px]] |
| - | + | ||
| - | '''Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution''' | + | {{Structure |
| + | |PDB= 2qzv |SIZE=350|CAPTION= <scene name='initialview01'>2qzv</scene>, resolution 9.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Mvp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | ||
| + | }} | ||
| + | |||
| + | '''Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2QZV is a [ | + | 2QZV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZV OCA]. |
==Reference== | ==Reference== | ||
| - | Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP., Mikyas Y, Makabi M, Raval-Fernandes S, Harrington L, Kickhoefer VA, Rome LH, Stewart PL, J Mol Biol. 2004 Nov 12;344(1):91-105. PMID:[http:// | + | Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP., Mikyas Y, Makabi M, Raval-Fernandes S, Harrington L, Kickhoefer VA, Rome LH, Stewart PL, J Mol Biol. 2004 Nov 12;344(1):91-105. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15504404 15504404] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: Rome, L H.]] | [[Category: Rome, L H.]] | ||
[[Category: Sievers, S A.]] | [[Category: Sievers, S A.]] | ||
| - | [[Category: barrel self-assembled from 96 | + | [[Category: barrel self-assembled from 96 stave]] |
[[Category: cytoplasm]] | [[Category: cytoplasm]] | ||
[[Category: innate immunity]] | [[Category: innate immunity]] | ||
| Line 26: | Line 35: | ||
[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:31:15 2008'' |
Revision as of 16:31, 20 March 2008
| |||||||
| , resolution 9.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | Mvp (Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution
Overview
The vault is a highly conserved ribonucleoprotein particle found in all higher eukaryotes. It has a barrel-shaped structure and is composed of the major vault protein (MVP); vault poly(ADP-ribose) polymerase (VPARP); telomerase-associated protein 1 (TEP1); and small untranslated RNA (vRNA). Although its strong conservation and high abundance indicate an important cellular role, the function of the vault is unknown. In humans, vaults have been implicated in multidrug resistance during chemotherapy. Recently, assembly of recombinant vaults has been established in insect cells expressing only MVP. Here, we demonstrate that co-expression of MVP with one or both of the other two vault proteins results in their co-assembly into regularly shaped vaults. Particles assembled from MVP with N-terminal peptide tags of various length are compared. Cryoelectron microscopy (cryoEM) and single-particle image reconstruction methods were used to determine the structure of nine recombinant vaults of various composition, as well as wild-type and TEP1-deficient mouse vaults. Recombinant vaults with MVP N-terminal peptide tags showed internal density that varied in size with the length of the tag. Reconstruction of a recombinant vault with a cysteine-rich tag revealed 48-fold rotational symmetry for the vault. A model is proposed for the organization of MVP within the vault with all of the MVP N termini interacting non-covalently at the vault midsection and 48 copies of MVP forming each half vault. CryoEM difference mapping localized VPARP to three density bands lining the inner surface of the vault. Difference maps designed to localize TEP1 showed only weak density inside of the caps, suggesting that TEP1 may interact with MVP via a small interaction region. In the absence of atomic-resolution structures for either VPARP or TEP1, fold recognition methods were applied. A total of 21 repeats were predicted for the TEP1 WD-repeat domain, suggesting an unusually large beta-propeller fold.
About this Structure
2QZV is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP., Mikyas Y, Makabi M, Raval-Fernandes S, Harrington L, Kickhoefer VA, Rome LH, Stewart PL, J Mol Biol. 2004 Nov 12;344(1):91-105. PMID:15504404
Page seeded by OCA on Thu Mar 20 18:31:15 2008
