2r0g

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[[Image:2r0g.jpg|left|200px]]<br /><applet load="2r0g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2r0g.jpg|left|200px]]
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caption="2r0g, resolution 2.37&Aring;" />
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'''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c'''<br />
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{{Structure
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|PDB= 2r0g |SIZE=350|CAPTION= <scene name='initialview01'>2r0g</scene>, resolution 2.37&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=7CK:7-carboxy-5-hydroxy-12,13-dihydro-6H-indolo[2,3-a]pyrrolo[3,4-c]carbazole'>7CK</scene>
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|ACTIVITY=
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|GENE= rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])
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}}
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'''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2R0G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=7CK:'>7CK</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0G OCA].
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2R0G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0G OCA].
==Reference==
==Reference==
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Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17873060 17873060]
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Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17873060 17873060]
[[Category: Lechevalieria aerocolonigenes]]
[[Category: Lechevalieria aerocolonigenes]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:43:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:31:25 2008''

Revision as of 16:31, 20 March 2008


PDB ID 2r0g

Drag the structure with the mouse to rotate
, resolution 2.37Å
Ligands: and
Gene: rbmD, rebC (Lechevalieria aerocolonigenes)
Coordinates: save as pdb, mmCIF, xml



Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c


Overview

The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to accelerate product formation and eliminate side reactions. However, the mode of action of RebC was largely unknown. Using crystallography, we have determined a likely function for RebC as a flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding pocket. These studies strongly suggest that the role of RebC is to sequester a reactive intermediate produced by its partner protein and to react with it enzymatically, preventing its conversion to a suite of degradation products that includes, at low levels, the desired product.

About this Structure

2R0G is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.

Reference

Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:17873060

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