2r4v
From Proteopedia
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| - | [[Image:2r4v.jpg|left|200px]] | + | [[Image:2r4v.jpg|left|200px]] |
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| - | '''Structure of human CLIC2, crystal form A''' | + | {{Structure |
| + | |PDB= 2r4v |SIZE=350|CAPTION= <scene name='initialview01'>2r4v</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GTT:GLUTATHIONE'>GTT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CLIC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of human CLIC2, crystal form A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2R4V is a [ | + | 2R4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R4V OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Janus protein human CLIC2., Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW, J Mol Biol. 2007 Nov 30;374(3):719-31. Epub 2007 Sep 20. PMID:[http:// | + | Structure of the Janus protein human CLIC2., Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW, J Mol Biol. 2007 Nov 30;374(3):719-31. Epub 2007 Sep 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17945253 17945253] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: GTT]] | [[Category: GTT]] | ||
[[Category: chloride channel]] | [[Category: chloride channel]] | ||
| - | [[Category: chloride intracellular | + | [[Category: chloride intracellular channel]] |
[[Category: clic2]] | [[Category: clic2]] | ||
[[Category: ion transport]] | [[Category: ion transport]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:32:34 2008'' |
Revision as of 16:32, 20 March 2008
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| , resolution 1.85Å | |||||||
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| Ligands: | |||||||
| Gene: | CLIC2 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of human CLIC2, crystal form A
Overview
Chloride intracellular channel (CLIC) proteins possess the remarkable property of being able to convert from a water-soluble state to a membrane channel state. We determined the three-dimensional structure of human CLIC2 in its water-soluble form by X-ray crystallography at 1.8-A resolution from two crystal forms. In contrast to the previously characterized CLIC1 protein, which forms a possibly functionally important disulfide-induced dimer under oxidizing conditions, we show that CLIC2 possesses an intramolecular disulfide and that the protein remains monomeric irrespective of redox conditions. Site-directed mutagenesis studies show that removal of the intramolecular disulfide or introduction of cysteine residues in CLIC2, equivalent to those that form the intramolecular disulfide in CLIC1, does not cause dimer formation under oxidizing conditions. We also show that CLIC2 forms pH-dependent chloride channels in vitro with higher channel activity at low pH levels and that the channels are subject to redox regulation. In both crystal forms, we observed an extended loop region from the C-terminal domain, called the foot loop, inserting itself into an interdomain crevice of a neighboring molecule. The equivalent region in the structurally related glutathione transferase superfamily corresponds to the active site. This so-called foot-in-mouth interaction suggests that CLIC2 might recognize other proteins such as the ryanodine receptor through a similar interaction.
About this Structure
2R4V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Janus protein human CLIC2., Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW, J Mol Biol. 2007 Nov 30;374(3):719-31. Epub 2007 Sep 20. PMID:17945253
Page seeded by OCA on Thu Mar 20 18:32:34 2008
Categories: Homo sapiens | Single protein | Cromer, B A. | Gorman, M A. | Hansen, G. | Parker, M W. | GTT | Chloride channel | Chloride intracellular channel | Clic2 | Ion transport | Ionic channel | Pore-forming protein | Ryanodine receptor | Transport | Transport protein | Voltage-gated channel | X-ray crystallography
