2r9z

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(New page: 200px<br /><applet load="2r9z" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r9z, resolution 2.10&Aring;" /> '''Glutathione amide re...)
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[[Image:2r9z.jpg|left|200px]]<br /><applet load="2r9z" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2r9z.jpg|left|200px]]
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caption="2r9z, resolution 2.10&Aring;" />
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'''Glutathione amide reductase from Chromatium gracile'''<br />
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{{Structure
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|PDB= 2r9z |SIZE=350|CAPTION= <scene name='initialview01'>2r9z</scene>, resolution 2.10&Aring;
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+464'>AC1</scene>, <scene name='pdbsite=AC2:Ni+Binding+Site+For+Residue+A+466'>AC2</scene>, <scene name='pdbsite=AC3:Cl+Binding+Site+For+Residue+B+464'>AC3</scene>, <scene name='pdbsite=AC4:Ni+Binding+Site+For+Residue+B+466'>AC4</scene>, <scene name='pdbsite=AC5:Ni+Binding+Site+For+Residue+A+468'>AC5</scene>, <scene name='pdbsite=AC6:Ni+Binding+Site+For+Residue+B+468'>AC6</scene>, <scene name='pdbsite=AC7:Fad+Binding+Site+For+Residue+A+500'>AC7</scene> and <scene name='pdbsite=AC8:Fad+Binding+Site+For+Residue+B+500'>AC8</scene>
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Glutathione amide reductase from Chromatium gracile'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2R9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Marichromatium_gracile Marichromatium gracile] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+464'>AC1</scene>, <scene name='pdbsite=AC2:Ni+Binding+Site+For+Residue+A+466'>AC2</scene>, <scene name='pdbsite=AC3:Cl+Binding+Site+For+Residue+B+464'>AC3</scene>, <scene name='pdbsite=AC4:Ni+Binding+Site+For+Residue+B+466'>AC4</scene>, <scene name='pdbsite=AC5:Ni+Binding+Site+For+Residue+A+468'>AC5</scene>, <scene name='pdbsite=AC6:Ni+Binding+Site+For+Residue+B+468'>AC6</scene>, <scene name='pdbsite=AC7:Fad+Binding+Site+For+Residue+A+500'>AC7</scene> and <scene name='pdbsite=AC8:Fad+Binding+Site+For+Residue+B+500'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Z OCA].
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2R9Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Marichromatium_gracile Marichromatium gracile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9Z OCA].
==Reference==
==Reference==
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Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase., Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J, J Mol Biol. 2007 Dec 7;374(4):883-9. Epub 2007 Sep 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17977556 17977556]
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Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase., Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J, J Mol Biol. 2007 Dec 7;374(4):883-9. Epub 2007 Sep 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17977556 17977556]
[[Category: Marichromatium gracile]]
[[Category: Marichromatium gracile]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: substrate specificity]]
[[Category: substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:33:58 2008''

Revision as of 16:34, 20 March 2008

Image:2r9z.jpg


PDB ID 2r9z

Drag the structure with the mouse to rotate
, resolution 2.10Å
Sites: , , , , , , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



Glutathione amide reductase from Chromatium gracile


Overview

Glutathione reductase (GR) plays a vital role in maintaining the antioxidant levels of the cytoplasm by catalyzing the reduction of glutathione disulfide to reduced glutathione, thereby using NADPH and flavin adenine dinucleotide as cofactors. Chromatiaceae have evolved an unusual homolog that prefers both a modified substrate (glutathione amide disulfide [GASSAG]) and a different cofactor (NADH). Herein, we present the crystal structure of the Chromatium gracile glutathione amide reductase (GAR) both alone and in complex with NAD(+). An altered charge distribution in the GASSAG binding pocket explains the difference in substrate specificity. The NADH binding pocket of GAR differs from that of wild-type GR as well as that of a low active GR that was engineered to mimic NADH binding. Based on the GAR structure, we propose two attractive rationales for producing an efficient GR enzyme with NADH specificity.

About this Structure

2R9Z is a Single protein structure of sequence from Marichromatium gracile. Full crystallographic information is available from OCA.

Reference

Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase., Van Petegem F, De Vos D, Savvides S, Vergauwen B, Van Beeumen J, J Mol Biol. 2007 Dec 7;374(4):883-9. Epub 2007 Sep 29. PMID:17977556

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