5af6

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'''Unreleased structure'''
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==Structure of Lys33-linked diUb bound to Trabid NZF1==
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<StructureSection load='5af6' size='340' side='right' caption='[[5af6]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5af6]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AF6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5af4|5af4]], [[5af5|5af5]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5af6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5af6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5af6 RCSB], [http://www.ebi.ac.uk/pdbsum/5af6 PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/ZRAN1_HUMAN ZRAN1_HUMAN]] Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling.<ref>PMID:18281465</ref> <ref>PMID:21834987</ref> <ref>PMID:23827681</ref> <ref>PMID:22157957</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein ubiquitination regulates many cellular processes via attachment of structurally and functionally distinct ubiquitin (Ub) chains. Several atypical chain types have remained poorly characterized because the enzymes mediating their assembly and receptors with specific binding properties have been elusive. We found that the human HECT E3 ligases UBE3C and AREL1 assemble K48/K29- and K11/K33-linked Ub chains, respectively, and can be used in combination with DUBs to generate K29- and K33-linked chains for biochemical and structural analyses. Solution studies indicate that both chains adopt open and dynamic conformations. We further show that the N-terminal Npl4-like zinc finger (NZF1) domain of the K29/K33-specific deubiquitinase TRABID specifically binds K29/K33-linked diUb, and a crystal structure of this complex explains TRABID specificity and suggests a model for chain binding by TRABID. Our work uncovers linkage-specific components in the Ub system for atypical K29- and K33-linked Ub chains, providing tools to further understand these unstudied posttranslational modifications.
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The entry 5af6 is ON HOLD until Paper Publication
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Assembly and Specific Recognition of K29- and K33-Linked Polyubiquitin.,Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D Mol Cell. 2015 Mar 4. pii: S1097-2765(15)00091-X. doi:, 10.1016/j.molcel.2015.01.042. PMID:25752577<ref>PMID:25752577</ref>
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Authors: Michel, M.A., Elliott, P.R., Swatek, K.N., Simicek, M., Pruneda, J.N., Wagstaff, J.L., Freund, S.M.V., Komander, D.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Structure of Lys33-linked diUb bound to Trabid NZF1
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Elliott, P.R]]
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__TOC__
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[[Category: Freund, S.M.V]]
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</StructureSection>
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[[Category: Wagstaff, J.L]]
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[[Category: Elliott, P R]]
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[[Category: Swatek, K.N]]
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[[Category: Freund, S M.V]]
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[[Category: Michel, M.A]]
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[[Category: Komander, D]]
[[Category: Komander, D]]
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[[Category: Michel, M A]]
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[[Category: Pruneda, J N]]
[[Category: Simicek, M]]
[[Category: Simicek, M]]
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[[Category: Pruneda, J.N]]
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[[Category: Swatek, K N]]
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[[Category: Wagstaff, J L]]
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[[Category: Signaling protein]]
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[[Category: Ubiquitin]]
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[[Category: Ubiquitin trabid]]

Revision as of 13:59, 26 March 2015

Structure of Lys33-linked diUb bound to Trabid NZF1

5af6, resolution 3.40Å

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