4u7q
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Structure of wild-type HIV protease in complex with photosensitive inhibitor PDI-6== |
| + | <StructureSection load='4u7q' size='340' side='right' caption='[[4u7q]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4u7q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U7Q FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3EM:N~2~-({[7-(DIETHYLAMINO)-2-OXO-2H-CHROMEN-4-YL]METHOXY}CARBONYL)-N-[(2S,4S,5S)-4-HYDROXY-1,6-DIPHENYL-5-{[(1,3-THIAZOL-5-YLMETHOXY)CARBONYL]AMINO}HEXAN-2-YL]-L-VALINAMIDE'>3EM</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4u7v|4u7v]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u7q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4u7q RCSB], [http://www.ebi.ac.uk/pdbsum/4u7q PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HIV protease (PR) is required for proteolytic maturation in the late phase of HIV replication and represents a prime therapeutic target. The regulation and kinetics of viral polyprotein processing and maturation are currently not understood in detail. Here we design, synthesize, validate and apply a potent, photodegradable HIV PR inhibitor to achieve synchronized induction of proteolysis. The compound exhibits subnanomolar inhibition in vitro. Its photolabile moiety is released on light irradiation, reducing the inhibitory potential by 4 orders of magnitude. We determine the structure of the PR-inhibitor complex, analyze its photolytic products, and show that the enzymatic activity of inhibited PR can be fully restored on inhibitor photolysis. We also demonstrate that proteolysis of immature HIV particles produced in the presence of the inhibitor can be rapidly triggered by light enabling thus to analyze the timing, regulation and spatial requirements of viral processing in real time. | ||
| - | + | Triggering HIV polyprotein processing by light using rapid photodegradation of a tight-binding protease inhibitor.,Schimer J, Pavova M, Anders M, Pachl P, Sacha P, Cigler P, Weber J, Majer P, Rezacova P, Krausslich HG, Muller B, Konvalinka J Nat Commun. 2015 Mar 9;6:6461. doi: 10.1038/ncomms7461. PMID:25751579<ref>PMID:25751579</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Pachl, P]] | [[Category: Pachl, P]] | ||
[[Category: Rezacova, P]] | [[Category: Rezacova, P]] | ||
| + | [[Category: Schimer, J]] | ||
| + | [[Category: Aspartic protease]] | ||
| + | [[Category: Hiv-1]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Inhibition]] | ||
| + | [[Category: Viral protease]] | ||
Revision as of 14:01, 26 March 2015
Structure of wild-type HIV protease in complex with photosensitive inhibitor PDI-6
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