2rcv

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(New page: 200px<br /><applet load="2rcv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rcv, resolution 1.60&Aring;" /> '''Crystal structure of...)
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[[Image:2rcv.jpg|left|200px]]<br /><applet load="2rcv" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2rcv.jpg|left|200px]]
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caption="2rcv, resolution 1.60&Aring;" />
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'''Crystal structure of the Bacillus subtilis superoxide dismutase'''<br />
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{{Structure
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|PDB= 2rcv |SIZE=350|CAPTION= <scene name='initialview01'>2rcv</scene>, resolution 1.60&Aring;
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|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Residue+G+203'>AC1</scene>, <scene name='pdbsite=AC2:Mn+Binding+Site+For+Residue+A+203'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+E+203'>AC3</scene>, <scene name='pdbsite=AC4:Mn+Binding+Site+For+Residue+F+203'>AC4</scene>, <scene name='pdbsite=AC5:Mn+Binding+Site+For+Residue+B+203'>AC5</scene>, <scene name='pdbsite=AC6:Mn+Binding+Site+For+Residue+C+203'>AC6</scene>, <scene name='pdbsite=AC7:Mn+Binding+Site+For+Residue+D+203'>AC7</scene> and <scene name='pdbsite=AC8:Mn+Binding+Site+For+Residue+H+203'>AC8</scene>
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|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]
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|GENE= sodA, yqgD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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}}
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'''Crystal structure of the Bacillus subtilis superoxide dismutase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2RCV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Known structural/functional Sites: <scene name='pdbsite=AC1:Mn+Binding+Site+For+Residue+G+203'>AC1</scene>, <scene name='pdbsite=AC2:Mn+Binding+Site+For+Residue+A+203'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+E+203'>AC3</scene>, <scene name='pdbsite=AC4:Mn+Binding+Site+For+Residue+F+203'>AC4</scene>, <scene name='pdbsite=AC5:Mn+Binding+Site+For+Residue+B+203'>AC5</scene>, <scene name='pdbsite=AC6:Mn+Binding+Site+For+Residue+C+203'>AC6</scene>, <scene name='pdbsite=AC7:Mn+Binding+Site+For+Residue+D+203'>AC7</scene> and <scene name='pdbsite=AC8:Mn+Binding+Site+For+Residue+H+203'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCV OCA].
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2RCV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCV OCA].
==Reference==
==Reference==
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Structure of Bacillus subtilis superoxide dismutase., Liu P, Ewis HE, Huang YJ, Lu CD, Tai PC, Weber IT, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1003-7. Epub 2007 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18084079 18084079]
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Structure of Bacillus subtilis superoxide dismutase., Liu P, Ewis HE, Huang YJ, Lu CD, Tai PC, Weber IT, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1003-7. Epub 2007 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18084079 18084079]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Weber, I T]]
[[Category: Weber, I T]]
[[Category: MN]]
[[Category: MN]]
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[[Category: bacillus subtilis]]
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[[Category: bacillus subtili]]
[[Category: manganese]]
[[Category: manganese]]
[[Category: metal-binding]]
[[Category: metal-binding]]
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[[Category: superoxide dismutase]]
[[Category: superoxide dismutase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:59:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:34:48 2008''

Revision as of 16:34, 20 March 2008

Image:2rcv.jpg


PDB ID 2rcv

Drag the structure with the mouse to rotate
, resolution 1.60Å
Sites: , , , , , , and
Ligands:
Gene: sodA, yqgD (Bacillus subtilis)
Activity: Superoxide dismutase, with EC number 1.15.1.1
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the Bacillus subtilis superoxide dismutase


Overview

The sodA gene of Bacillus subtilis was expressed in Escherichia coli, purified and crystallized. The crystal structure of MnSOD was solved by molecular replacement with four dimers per asymmetric unit and refined to an R factor of 21.1% at 1.8 A resolution. The dimer structure is very similar to that of the related enzyme from B. anthracis. Larger structural differences were observed with the human MnSOD, which has one less helix in the helical domain and a longer loop between two beta-strands and also showed differences in three amino acids at the intersubunit interface in the dimer compared with the two bacterial MnSODs. These structural differences can be exploited in the design of drugs that selectively target the Bacillus enzymes.

About this Structure

2RCV is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure of Bacillus subtilis superoxide dismutase., Liu P, Ewis HE, Huang YJ, Lu CD, Tai PC, Weber IT, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1003-7. Epub 2007 Nov 21. PMID:18084079

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