2rdl
From Proteopedia
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| - | [[Image:2rdl.gif|left|200px]] | + | [[Image:2rdl.gif|left|200px]] |
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| - | '''Hamster Chymase 2''' | + | {{Structure |
| + | |PDB= 2rdl |SIZE=350|CAPTION= <scene name='initialview01'>2rdl</scene>, resolution 2.500Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Msu+Binding+Site+For+Residue+I+1'>AC1</scene>, <scene name='pdbsite=AC2:Msu+Binding+Site+For+Residue+J+1'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+246'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+B+246'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+247'>AC5</scene> and <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+B+247'>AC6</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Hamster Chymase 2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2RDL is a [ | + | 2RDL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDL OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for elastolytic substrate specificity in rodent alpha-chymases., Kervinen J, Abad M, Crysler C, Kolpak M, Mahan AD, Masucci JA, Bayoumy S, Cummings MD, Yao X, Olson M, de Garavilla L, Kuo L, Deckman I, Spurlino J, J Biol Chem. 2008 Jan 4;283(1):427-36. Epub 2007 Oct 31. PMID:[http:// | + | Structural basis for elastolytic substrate specificity in rodent alpha-chymases., Kervinen J, Abad M, Crysler C, Kolpak M, Mahan AD, Masucci JA, Bayoumy S, Cummings MD, Yao X, Olson M, de Garavilla L, Kuo L, Deckman I, Spurlino J, J Biol Chem. 2008 Jan 4;283(1):427-36. Epub 2007 Oct 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17981788 17981788] |
[[Category: Mesocricetus auratus]] | [[Category: Mesocricetus auratus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:03 2008'' |
Revision as of 16:35, 20 March 2008
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| , resolution 2.500Å | |||||||
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| Sites: | , , , , and | ||||||
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hamster Chymase 2
Overview
Divergence of substrate specificity within the context of a common structural framework represents an important mechanism by which new enzyme activity naturally evolves. We present enzymological and x-ray structural data for hamster chymase-2 (HAM2) that provides a detailed explanation for the unusual hydrolytic specificity of this rodent alpha-chymase. In enzymatic characterization, hamster chymase-1 (HAM1) showed typical chymase proteolytic activity. In contrast, HAM2 exhibited atypical substrate specificity, cleaving on the carboxyl side of the P1 substrate residues Ala and Val, characteristic of elastolytic rather than chymotryptic specificity. The 2.5-A resolution crystal structure of HAM2 complexed to the peptidyl inhibitor MeOSuc-Ala-Ala-Pro-Ala-chloromethylketone revealed a narrow and shallow S1 substrate binding pocket that accommodated only a small hydrophobic residue (e.g. Ala or Val). The different substrate specificities of HAM2 and HAM1 are explained by changes in four S1 substrate site residues (positions 189, 190, 216, and 226). Of these, Asn(189), Val(190), and Val(216) form an easily identifiable triplet in all known rodent alpha-chymases that can be used to predict elastolytic specificity for novel chymase-like sequences. Phylogenetic comparison defines guinea pig and rabbit chymases as the closest orthologs to rodent alpha-chymases.
About this Structure
2RDL is a Single protein structure of sequence from Mesocricetus auratus. Full crystallographic information is available from OCA.
Reference
Structural basis for elastolytic substrate specificity in rodent alpha-chymases., Kervinen J, Abad M, Crysler C, Kolpak M, Mahan AD, Masucci JA, Bayoumy S, Cummings MD, Yao X, Olson M, de Garavilla L, Kuo L, Deckman I, Spurlino J, J Biol Chem. 2008 Jan 4;283(1):427-36. Epub 2007 Oct 31. PMID:17981788
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