2rds
From Proteopedia
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| - | [[Image:2rds.gif|left|200px]] | + | [[Image:2rds.gif|left|200px]] |
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| - | '''Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound''' | + | {{Structure |
| + | |PDB= 2rds |SIZE=350|CAPTION= <scene name='initialview01'>2rds</scene>, resolution 1.650Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OGA:N-OXALYOLGLYCINE'>OGA</scene> and <scene name='pdbligand=1PL:(1S,3aS,5aR,8aS)-1,7,7-trimethyl-1,2,3,3a,5a,6,7,8-octahydrocyclopenta[c]pentalene-4-carboxylic acid'>1PL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= ptlH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33903 Streptomyces avermitilis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2RDS is a [ | + | 2RDS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avermitilis Streptomyces avermitilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDS OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis., You Z, Omura S, Ikeda H, Cane DE, Jogl G, J Biol Chem. 2007 Dec 14;282(50):36552-60. Epub 2007 Oct 16. PMID:[http:// | + | Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis., You Z, Omura S, Ikeda H, Cane DE, Jogl G, J Biol Chem. 2007 Dec 14;282(50):36552-60. Epub 2007 Oct 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17942405 17942405] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces avermitilis]] | [[Category: Streptomyces avermitilis]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:04 2008'' |
Revision as of 16:35, 20 March 2008
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| , resolution 1.650Å | |||||||
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| Ligands: | , , and | ||||||
| Gene: | ptlH (Streptomyces avermitilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound
Overview
The non-heme iron dioxygenase PtlH from the soil organism Streptomyces avermitilis is a member of the iron(II)/alpha-ketoglutarate-dependent dioxygenase superfamily and catalyzes an essential reaction in the biosynthesis of the sesquiterpenoid antibiotic pentalenolactone. To investigate the structural basis for substrate recognition and catalysis, we have determined the x-ray crystal structure of PtlH in several complexes with the cofactors iron, alpha-ketoglutarate, and the non-reactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold, and the cofactor-binding site for iron and alpha-ketoglutarate is similar to other double-stranded barrel helix fold enzymes. Additional secondary structure elements that contribute to the substrate-binding site in PtlH are not conserved in other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate. Kinetic analysis of wild type and site-directed mutant proteins confirms a critical function of two arginine residues in substrate binding, while simulated docking of the enzymatic reaction product reveals the likely orientation of bound substrate.
About this Structure
2RDS is a Single protein structure of sequence from Streptomyces avermitilis. Full crystallographic information is available from OCA.
Reference
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis., You Z, Omura S, Ikeda H, Cane DE, Jogl G, J Biol Chem. 2007 Dec 14;282(50):36552-60. Epub 2007 Oct 16. PMID:17942405
Page seeded by OCA on Thu Mar 20 18:35:04 2008
Categories: Single protein | Streptomyces avermitilis | Cane, D E. | Ikeda, H. | Jogl, G. | Omura, S. | You, Z. | 1PL | FE | MG | OGA | Dioxygenase | Double stranded barrel helix | Oxidoreductase
