2rg8

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(New page: 200px<br /><applet load="2rg8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rg8, resolution 1.8&Aring;" /> '''Crystal Structure of ...)
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[[Image:2rg8.jpg|left|200px]]<br /><applet load="2rg8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2rg8.jpg|left|200px]]
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caption="2rg8, resolution 1.8&Aring;" />
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'''Crystal Structure of Programmed for Cell Death 4 Middle MA3 domain'''<br />
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{{Structure
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|PDB= 2rg8 |SIZE=350|CAPTION= <scene name='initialview01'>2rg8</scene>, resolution 1.8&Aring;
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+2'>AC2</scene>, <scene name='pdbsite=AC3:Na+Binding+Site+For+Residue+B+3'>AC3</scene>, <scene name='pdbsite=AC4:Na+Binding+Site+For+Residue+A+4'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Residue+B+5'>AC5</scene>
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
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|ACTIVITY=
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|GENE= PDCD4, H731 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal Structure of Programmed for Cell Death 4 Middle MA3 domain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2RG8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Residue+B+2'>AC2</scene>, <scene name='pdbsite=AC3:Na+Binding+Site+For+Residue+B+3'>AC3</scene>, <scene name='pdbsite=AC4:Na+Binding+Site+For+Residue+A+4'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Residue+B+5'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RG8 OCA].
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2RG8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RG8 OCA].
==Reference==
==Reference==
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PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains., Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G, Proc Natl Acad Sci U S A. 2008 Feb 22;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18296639 18296639]
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PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains., Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G, Proc Natl Acad Sci U S A. 2008 Feb 22;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18296639 18296639]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: translation]]
[[Category: translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 5 13:22:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:49 2008''

Revision as of 16:35, 20 March 2008

Image:2rg8.jpg


PDB ID 2rg8

Drag the structure with the mouse to rotate
, resolution 1.8Å
Sites: , , , and
Ligands: and
Gene: PDCD4, H731 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Programmed for Cell Death 4 Middle MA3 domain


Overview

Programmed Cell Death 4 (PDCD4) is a protein known to bind eukaryotic initiation factor 4A (eIF4A), inhibit translation initiation, and act as a tumor suppressor. PDCD4 contains two C-terminal MA3 domains, which are thought to be responsible for its inhibitory function. Here, we analyze the structures and inhibitory functions of these two PDCD4 MA3 domains by x-ray crystallography, NMR, and surface plasmon resonance. We show that both MA3 domains are structurally and functionally very similar and bind specifically to the eIF4A N-terminal domain (eIF4A-NTD) using similar binding interfaces. We found that the PDCD4 MA3 domains compete with the eIF4G MA3 domain and RNA for eIF4A binding. Our data provide evidence that PDCD4 inhibits translation initiation by displacing eIF4G and RNA from eIF4A. The PDCD4 MA3 domains act synergistically to form a tighter and more stable complex with eIF4A, which explains the need for two tandem MA3 domains.

About this Structure

2RG8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains., Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G, Proc Natl Acad Sci U S A. 2008 Feb 22;. PMID:18296639

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