2rji
From Proteopedia
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| - | [[Image:2rji.gif|left|200px]] | + | [[Image:2rji.gif|left|200px]] |
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| - | '''Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface''' | + | {{Structure |
| + | |PDB= 2rji |SIZE=350|CAPTION= <scene name='initialview01'>2rji</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= eba175reVIsynth ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | ||
| + | }} | ||
| + | |||
| + | '''Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2RJI is a [ | + | 2RJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJI OCA]. |
==Reference== | ==Reference== | ||
| - | Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface., Withers-Martinez C, Haire LF, Hackett F, Walker PA, Howell SA, Smerdon SJ, Dodson GG, Blackman MJ, J Mol Biol. 2008 Jan 18;375(3):773-81. Epub 2007 Nov 1. PMID:[http:// | + | Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface., Withers-Martinez C, Haire LF, Hackett F, Walker PA, Howell SA, Smerdon SJ, Dodson GG, Blackman MJ, J Mol Biol. 2008 Jan 18;375(3):773-81. Epub 2007 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18036613 18036613] |
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: region vi]] | [[Category: region vi]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:36:45 2008'' |
Revision as of 16:36, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Ligands: | |||||||
| Gene: | eba175reVIsynth (Plasmodium falciparum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface
Overview
The malaria parasite proliferates in the bloodstream of its vertebrate host by invading and replicating within erythrocytes. To achieve successful invasion, a number of discrete and essential events need to take place at the parasite-host cell interface. Erythrocyte-binding antigen 175 (EBA-175) is a member of a family of Plasmodium falciparum erythrocyte-binding proteins involved in the formation of a tight junction, a necessary step in invasion. Here we present the crystal structure of EBA-175 region VI (rVI), a cysteine-rich domain that is highly conserved within the protein family and is essential for EBA-175 trafficking. The structure was solved by selenomethionine single-wavelength anomalous dispersion at 1.8 A resolution. It reveals a homodimer, containing in each subunit a compact five-alpha-helix core that is stabilized by four conserved disulfide bridges. rVI adopts a novel fold that is likely conserved across the protein family, indicating a conserved function. It shows no similarity to the Duffy-binding-like domains of EBA-175 involved in erythrocyte binding, indicating a distinct role. Remarkably, rVI possesses structural features related to the KIX-binding domain of the coactivator CREB-binding protein, supporting the binding and trafficking roles that have been ascribed to it and providing a rational basis for further experimental investigation of its function.
About this Structure
2RJI is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Malarial EBA-175 region VI crystallographic structure reveals a KIX-like binding interface., Withers-Martinez C, Haire LF, Hackett F, Walker PA, Howell SA, Smerdon SJ, Dodson GG, Blackman MJ, J Mol Biol. 2008 Jan 18;375(3):773-81. Epub 2007 Nov 1. PMID:18036613
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