2rlu
From Proteopedia
(New page: 200px<br /><applet load="2rlu" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rlu" /> '''The Three Dimensional Structure of the Moore...) |
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| - | '''The Three Dimensional Structure of the Moorella thermoacetica Selenocysteine Insertion Sequence RNA Hairpin and its Interaction with the Elongation factor SelB''' | + | {{Structure |
| + | |PDB= 2rlu |SIZE=350|CAPTION= <scene name='initialview01'>2rlu</scene> | ||
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| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
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| + | '''The Three Dimensional Structure of the Moorella thermoacetica Selenocysteine Insertion Sequence RNA Hairpin and its Interaction with the Elongation factor SelB''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2RLU is a [ | + | 2RLU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RLU OCA]. |
==Reference== | ==Reference== | ||
| - | The three-dimensional structure of the Moorella thermoacetica selenocysteine insertion sequence RNA hairpin and its interaction with the elongation factor SelB., Beribisky AV, Tavares TJ, Amborski AN, Motamed M, Johnson AE, Mark TL, Johnson PE, RNA. 2007 Nov;13(11):1948-56. Epub 2007 Sep 27. PMID:[http:// | + | The three-dimensional structure of the Moorella thermoacetica selenocysteine insertion sequence RNA hairpin and its interaction with the elongation factor SelB., Beribisky AV, Tavares TJ, Amborski AN, Motamed M, Johnson AE, Mark TL, Johnson PE, RNA. 2007 Nov;13(11):1948-56. Epub 2007 Sep 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17901155 17901155] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Amborski, A N.]] | [[Category: Amborski, A N.]] | ||
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[[Category: Tavares, T J.]] | [[Category: Tavares, T J.]] | ||
[[Category: nmr]] | [[Category: nmr]] | ||
| - | [[Category: protein-rna | + | [[Category: protein-rna interaction]] |
[[Category: rna strcuture]] | [[Category: rna strcuture]] | ||
[[Category: selb]] | [[Category: selb]] | ||
[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:37:24 2008'' |
Revision as of 16:37, 20 March 2008
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The Three Dimensional Structure of the Moorella thermoacetica Selenocysteine Insertion Sequence RNA Hairpin and its Interaction with the Elongation factor SelB
Overview
Incorporation of the amino acid selenocysteine into a growing protein chain involves the interaction between a hairpin in the mRNA termed the selenocysteine insertion sequence (SECIS) and the special elongation factor SelB. Here we present the structure of the SECIS from the thermophilic organism Moorella thermoacetica (SECIS-MT) determined using nuclear magnetic resonance (NMR) spectroscopy. The SECIS-MT hairpin structure contains a pentaloop with the first and fourth nucleotides of the loop forming a noncanonical GC base pair; the fifth loop nucleotide is bulged out and unstructured. The G and U in positions two and three are on opposite sides of the loop and solvent exposed. The backbone resonances of the SECIS-binding domain from the M. thermoacetica SelB protein were assigned, and the degree of chemical shift perturbations that occur upon SECIS binding were mapped onto the structure of the complex. We demonstrate that a region in the third winged-helix domain of SelB, not previously implicated in binding, is affected by SECIS binding.
About this Structure
2RLU is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of the Moorella thermoacetica selenocysteine insertion sequence RNA hairpin and its interaction with the elongation factor SelB., Beribisky AV, Tavares TJ, Amborski AN, Motamed M, Johnson AE, Mark TL, Johnson PE, RNA. 2007 Nov;13(11):1948-56. Epub 2007 Sep 27. PMID:17901155
Page seeded by OCA on Thu Mar 20 18:37:24 2008
