2shk

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[[Image:2shk.jpg|left|200px]]<br /><applet load="2shk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2shk.jpg|left|200px]]
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caption="2shk, resolution 2.6&Aring;" />
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP'''<br />
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{{Structure
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|PDB= 2shk |SIZE=350|CAPTION= <scene name='initialview01'>2shk</scene>, resolution 2.6&Aring;
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|SITE= <scene name='pdbsite=PIP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]
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|GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])
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}}
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2SHK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] Known structural/functional Sites: <scene name='pdbsite=PIP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type-A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SHK OCA].
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2SHK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SHK OCA].
==Reference==
==Reference==
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Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299895 15299895]
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Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299895 15299895]
[[Category: Erwinia chrysanthemi]]
[[Category: Erwinia chrysanthemi]]
[[Category: Shikimate kinase]]
[[Category: Shikimate kinase]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:38:24 2008''

Revision as of 16:38, 20 March 2008

Image:2shk.jpg


PDB ID 2shk

Drag the structure with the mouse to rotate
, resolution 2.6Å
Sites: , , and
Ligands: and
Gene: AROL (Erwinia chrysanthemi)
Activity: Shikimate kinase, with EC number 2.7.1.71
Coordinates: save as pdb, mmCIF, xml



THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI COMPLEXED WITH ADP


Overview

Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

About this Structure

2SHK is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895

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