2sob
From Proteopedia
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| - | [[Image:2sob.gif|left|200px]] | + | [[Image:2sob.gif|left|200px]] |
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| - | '''SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES''' | + | {{Structure |
| + | |PDB= 2sob |SIZE=350|CAPTION= <scene name='initialview01'>2sob</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] | ||
| + | |GENE= STAPHYLOCOCCAL NUCLEASE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | ||
| + | }} | ||
| + | |||
| + | '''SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2SOB is a [ | + | 2SOB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. This structure supersedes the now removed PDB entry 1SOB. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SOB OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease., Alexandrescu AT, Gittis AG, Abeygunawardana C, Shortle D, J Mol Biol. 1995 Jul 7;250(2):134-43. PMID:[http:// | + | NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease., Alexandrescu AT, Gittis AG, Abeygunawardana C, Shortle D, J Mol Biol. 1995 Jul 7;250(2):134-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7608966 7608966] |
[[Category: Micrococcal nuclease]] | [[Category: Micrococcal nuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase (phosphoric diester)]] | [[Category: hydrolase (phosphoric diester)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:38:39 2008'' |
Revision as of 16:38, 20 March 2008
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| Gene: | STAPHYLOCOCCAL NUCLEASE (Staphylococcus aureus) | ||||||
| Activity: | Micrococcal nuclease, with EC number 3.1.31.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES
Overview
Similar folds often occur in proteins with dissimilar sequences. The OB-fold forms a part of the structures of at least seven non-homologous proteins that share either oligonucleotide or oligosaccharide binding functions. A 1-103 fragment corresponding to the OB-fold of the 149 amino acid residue staphylococcal nuclease gives NMR spectra characteristic of an unfolded protein, i.e. the wild-type nuclease sequence is insufficient to maintain a stable tertiary structure in the absence of the C-terminal one-third of this single-domain protein. By contrast, the 1-103 fragment of nuclease with the mutations Val66Leu and Gly88Val adopts a stable tertiary structure. The NMR solution structure of this latter fragment is a close variation of the OB-fold found in the X-ray structure of the parent protein. The Val66Leu and Gly88Val mutations appear to stabilize tertiary structure by consolidating the hydrophobic core of the nuclease OB-fold sub-domain. Taken together, these results suggest that recurrent structural motifs such as the OB-fold may in some cases represent vestiges of autonomous folding units that, during evolution, have become integrated into more complex cooperative folding domains.
About this Structure
2SOB is a Single protein structure of sequence from Staphylococcus aureus. This structure supersedes the now removed PDB entry 1SOB. Full crystallographic information is available from OCA.
Reference
NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease., Alexandrescu AT, Gittis AG, Abeygunawardana C, Shortle D, J Mol Biol. 1995 Jul 7;250(2):134-43. PMID:7608966
Page seeded by OCA on Thu Mar 20 18:38:39 2008
